Original Article
Clinical utility value of urinary aldosterone detection by tandem mass spectrometry in primary hyperaldosteronism screening
Wenjun Ma, Jin Bian, Ying Lou, Xu Yang, Huimin Zhang, Xianliang Zhou, Lei Song, Jun Cai, Beibei Zhao, Ergang Jiang, Weixia Liu, Yating Cheng
Published 2020-03-11
Cite as Chin J Lab Med, 2020, 43(3): 261-266. DOI: 10.3760/cma.j.issn.1009-9158.2020.03.013
Abstract
ObjectiveTo explore the clinical utility of liquid chromatography tandem mass spectrometry forprimary aldosteronism screening.
MethodsFrom January to October 2019, 413 inpatients diagnosed hypertension from Fuwai Hospital of Chinese Academy of Medical Sciences were enrolled, including 60 Primary aldosteronism(PA)patients and 353 primary hypertension patients. The plasma aldosterone concentration (PAC) and renin concentration (DRC) were measured after 2 h of standing. The 24 h urine samples were collected for measurement of aldosterone using LC-MS/MS. The performance of urine aldosterone and urine aldosterone/renin ratio (UADRR) in PA screening was evaluated by ROC, and compared with PAC/DRC ratio (ADRR). Meanwhile, the efficiency of urine aldosterone in elderly patients or patients with low blood potassium or 24 h urine sodium over 200 mmol was investigated.
ResultsArea under the curve (AUC)of urine aldosterone was 0.725 (95%CI 0.679-0.767), and the best cut-off was 7.13 μg/24 h, which was lower than AUC of ADRR (0.958, 95%CI 0.934-0.975). The AUC of UADRR was 0.947 (95%CI 0.920-0.966), the best cut-off was 1.11 (μg/24 h)/(μIU/ml), the sensitivity and specificity were 91.7% and 89.0%, respectively. There is no significant differences found with ADRR. In patients with 24 h urine sodium over 200 mmol, AUC of aldosterone was 0.834 (95%CI 0.730-0.910) and the best cut-off was 9.31 μg/24 h. The sensitivity and specificity were 90.9% and 68.7%, respectively. For the elderly patients over 60 years old, the AUC of urinary aldosterone was 0.860 (95%CI 0.770-0.925), and the best cut-off was 6.91 μg/24 h. The sensitivity and specificity were 84.6% and 81.3%, respectively. When admission blood potassium was less than 3.50 mmol/L, AUC of urinary aldosterone was 0.822 (95%CI 0.684-0.917), and the best cut-off was 10.63 μg/24 h. The sensitivity and specificity were 85.7% and 66.7%, respectively.
ConclusionThe detection of aldosterone in urine by LC-MS/MS can provide clinical information for PA screening, and the screening performance is better in patients with 24-hour urine sodium over 200 mmol, elderly patients or patients with low blood potassium. If combined with renin, screening efficiency was the same as that in ADRR.
Key words:
Hyperaldosteronism; Aldosterone; Urinalysis; Tandem mass spectrometry; Chromatography, liquid
Contributor Information
Wenjun Ma
Peking Union Medical College, National Cardiovascular Center of Chinese Academy of Medical Sciences, Hypertension Center of Fuwai Hospital, Beijing 100037, China
Jin Bian
Peking Union Medical College, National Cardiovascular Center of Chinese Academy of Medical Sciences, Hypertension Center of Fuwai Hospital, Beijing 100037, China
Ying Lou
Peking Union Medical College, National Cardiovascular Center of Chinese Academy of Medical Sciences, Hypertension Center of Fuwai Hospital, Beijing 100037, China
Xu Yang
Peking Union Medical College, National Cardiovascular Center of Chinese Academy of Medical Sciences, Hypertension Center of Fuwai Hospital, Beijing 100037, China
Huimin Zhang
Peking Union Medical College, National Cardiovascular Center of Chinese Academy of Medical Sciences, Hypertension Center of Fuwai Hospital, Beijing 100037, China
Xianliang Zhou
Peking Union Medical College, National Cardiovascular Center of Chinese Academy of Medical Sciences, Hypertension Center of Fuwai Hospital, Beijing 100037, China
Lei Song
Peking Union Medical College, National Cardiovascular Center of Chinese Academy of Medical Sciences, Hypertension Center of Fuwai Hospital, Beijing 100037, China
Jun Cai
Peking Union Medical College, National Cardiovascular Center of Chinese Academy of Medical Sciences, Hypertension Center of Fuwai Hospital, Beijing 100037, China
Beibei Zhao
Kingmed Laboratory test College of Guangzhou Medical University, Guangzhou Kingmed Diagnostics Group Co. Ltd, Guangzhou 510005, China
Ergang Jiang
Kingmed Laboratory test College of Guangzhou Medical University, Guangzhou Kingmed Diagnostics Group Co. Ltd, Guangzhou 510005, China
Weixia Liu
Kingmed Laboratory test College of Guangzhou Medical University, Guangzhou Kingmed Diagnostics Group Co. Ltd, Guangzhou 510005, China
Yating Cheng
Kingmed Laboratory test College of Guangzhou Medical University, Guangzhou Kingmed Diagnostics Group Co. Ltd, Guangzhou 510005, China
