Original Article
Application of element-tagged immunoassay combined with inductively coupled plasma mass spectrometry in HCG detection
Pu Danna, Jiang Wencan, Wen Xinyu, Guo Guanghong, Li Ping, Wang Chengbin
Published 2020-03-11
Cite as Chin J Lab Med, 2020, 43(3): 284-290. DOI: 10.3760/cma.j.issn.1009-9158.2020.03.017
Abstract
ObjectiveTo establish an inductively coupled plasma mass spectrometry (ICP-MS) based immunoassay method for the quantitative detection of human chorionic gonadotropin (HCG), and evaluate the clinical applicability of this method.
MethodsSm was selected as element tags, and the HCG quantitative detection system was established by double antibody sandwich method. The dosage of biotinylated antibody and reaction time were optimized. According to EP documents of Clinical and Laboratory Standards Institute (CLSI) and related standards, the analytical performance was evaluated after the establishment of the assay, including the limit of blank (LOB), linearity, precision, recovery, cross reactivity and interference test. And 88 clinical samples were measured using the new method compared to the electrochemical immunoassay (ECLIA) method.
ResultsTotal process completed within 30 min after optimization, and the optimal biotinylated antibody dosage was 0.5 μl. The LOB was 0.29 mIU/ml. The linearity was good within the range of 1.16-8 365.62 mIU/ml with the linear correlation coefficient greater than 0.995 (R2=0.998 0), the recovery was 97.53%-102.01%. Both intra-and inter-assay coefficients of variation of the high-value sample and the low-value sample were less than 10%. And there was no significant cross-reaction with Luteinizing hormone (LH), follicular stimulating hormone (FSH), and thyroid-stimulating hormone (TSH). The interference bias caused by different concentrations of interference substances was less than 10%. When compared with the ECLIA method for clinical sample detection, the proposed method showed a significant correlation(R2=0.960 0).
ConclusionThe proposed ICP-MS base immunoassay for HCG detection has good accuracy, high sensitivity and specificity, and the results of analytical performance verification meet the clinical requirements, which provides experimental basis for the clinical application of this method.
Key words:
Chorionic gonadotropin; Immunoassay; Spectrophotometry, atomic; Mass spectrometry
Contributor Information
Pu Danna
Hebei North University, Zhangjiakou 075000, China
Jiang Wencan
Center of Laboratory Medicine, the 1st Medical Center of PLA General Hospital, Beijing 100853, China
Wen Xinyu
Center of Laboratory Medicine, the 1st Medical Center of PLA General Hospital, Beijing 100853, China
Guo Guanghong
Center of Laboratory Medicine, the 1st Medical Center of PLA General Hospital, Beijing 100853, China
Li Ping
Hebei North University, Zhangjiakou 075000, China
Wang Chengbin
Center of Laboratory Medicine, the 1st Medical Center of PLA General Hospital, Beijing 100853, China