Experimental Research Article
Differential expression of miR-146a-5p in giant congenital melanocytic nevi, medium-sized congenital melanocytic nevi and normal skin
Min Sun, Xingang Wang, Zhuo Song, Ningbei Yin, Zhenmin Zhao, Di Wu
Published 2018-09-25
Cite as Chin J Plast Surg, 2018, 34(9): 747-751. DOI: 10.3760/cma.j.issn.1009-4598.2018.09.015
Abstract
ObjectiveTo investigate the differences in miRNA expression levels between giant congenital melanocytic nevi, medium-sized congenital melanocytic nevi and normal skin.
MethodsThe experiment was divided into three groups: giant congenital melanocytic nevi group, medium-sized congenital melanocytic nevi group and normal skin group, with ten samples in each group. Firstly, 3 samples of each group were detected by Agilent miRN Amicroarray to screen the different miRNAs between different groups. 5 differential miRNAs related to MAPK, Wnt, NF-kB signaling pathways were selected for further verification: miR-146a-5p, miR-140-5p, miR-106b-5p, miR-17-5p, and miR-483-5p. miRNA expression levels were measured using real-time quantitative PCR (Taqman) in ten clinical samples from each group. .Experimental data were analyzed using SPSS 22.0.
ResultsA total of 23 differential miRNAs between congenital melanocytic nevi and normal skin were found by miRNA microarray detection. The results of real-time PCR showed that miR-146a-5p expression was significantly different between the three groups: giant congenital melanocytic nevi VS medium-sized congenital melanocytic nevi (P=0.003); giant congenital melanocytic nevi VS normal skin (P=0.000); medium-sized congenital melanocytic nevi VS normal skin (P=0.013). There was no statistically significant difference in the expression of the other four miRNAs between the 3 groups.
ConclusionsThe expression of miR-146a-5p is increased in congenital melanocytic nevi, especially in giant congenital melanocytic nevi, which may be related to the proliferation and senescence of melanocytes in different tissues.
Key words:
Nevus pigmented; Melanocyte; microRNAs
Contributor Information
Min Sun
Department of Cleft Lip and Palate, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, China
Xingang Wang
Zhuo Song
Ningbei Yin
Zhenmin Zhao
Di Wu