Pediatrics Emergency and Critically ILL
The correlation between the apolipoprotein E genetic polymorphisms and sepsis in children
Liu Meixiu, Yang Changsheng, Li Qiang, He Leiyan, Fu Pan, Wang Aimin, Wang Chuanqing
Published 2017-06-10
Cite as Chin J Emerg Med, 2017,26(06): 622-626. DOI: 10.3760/cma.j.issn.1671-0282.2017.06.004
Abstract
ObjectiveTo study the correlation between apolipoprotein E (APOE) genetic polymorphisms and sepsis in Chinese children.
MethodsThe inpatients suffered with sepsis were enrolled as septic group and the healthy children from child health division were enrolled as control group. The study of APOE genotypes were carried out by polymerase chain reactions followed a high-resolution melting curve analysis. SPSS 16.0 statistical software was used for data analysis. Mann-Whitney U test was used to compare the age between the groups. Hardy-Weinberg equilibrium was tested using the Pearson χ2 -test. The χ2 -test was used to compare gender and the genotype distribution between the groups. The odd ratio (OR) was calculated together with its 95% confidence interval (CI). Potential confounding effects of variables were corrected using a multivariate unconditional logistic regression model. All statistical tests were two-sided and P < 0.05 indicates statistically significance.
ResultsAmong a total of 285 children collected from March 2011 to June 2012, there were 88 patients with sepsis and 197 healthy children. In the septic group, 15 septic patients were complicated with central nervous system infection. Four apolipoprotein E genotypes were identified to be ε3/ε3, ε2/ε3, ε3/ε4, and ε2/ε4. The percentage of each genotype found in patients of the septic group and the control group was 64.4% vs. 73.1% (ε3/ε3); 16.8% vs. 10.7% (ε2/ε3); 18.8% vs. 14.7% (ε3/ε4); 0% vs. 1.5% (ε2/ε4), respectively. The number of patients with the genotype ε3/ε3 among septic patients was significantly lower than that among the control individuals (P = 0.047, 1-β = 0.334, OR = 0.585, adjusted OR = 0.559). The number of patients with the genotype ε3/ε3 among the septic patients with central nervous system infection was 33.3%, which was also significantly lower than that among the septic patients without CNS infection (67.1%). (P = 0.014, 1-β = 0.685, OR = 0.245, adjusted OR = 0.275).
ConclusionsApolipoprotein E genetic polymorphisms were associated with the occurrence of sepsis and central nervous system complications in children. The susceptibility of children with genotype ε3/ε3 to sepsis and central nerve system infection complications is significantly lower than that of children with other genotypes.
Key words:
Apolipoprotein E; Polymorphism; Correlation; Sepsis; Central nerve system infection; High-resolution melting curve analysis; Children; China
Contributor Information
Liu Meixiu
The Department of Clinical Laboratory, Children’s Hospital of Fudan University, Shanghai 201199, China
Yang Changsheng
The Department of Cardiology, Zhongshan Hospital of Fudan University, Shanghai 200032, China
Li Qiang
Translational Medical Center for Development and Disease, Institute of Pediatrics, Children’s Hospital of Fudan University, Shanghai Key Laboratory of Birth Defect, Shanghai 201199, China
He Leiyan
The Department of Clinical Laboratory, Children’s Hospital of Fudan University, Shanghai 201199, China
Fu Pan
The Department of Clinical Laboratory, Children’s Hospital of Fudan University, Shanghai 201199, China
Wang Aimin
The Department of Clinical Laboratory, Children’s Hospital of Fudan University, Shanghai 201199, China
Wang Chuanqing
The Department of Nosocomial Infection Control, Children’s Hospital of Fudan University, Shanghai, 201199, China
