Acute Respiratory Distress Syndrome
The role of integrin involved heparin-binding protein secretion in acute lung injury
Yang Liu, Shaolin Ma, Xuebin Wang, Wei Xu, Shouqin Zhang, Xiangyu Zhang
Published 2018-07-10
Cite as Chin J Emerg Med, 2018, 27(7): 764-768. DOI: 10.3760/cma.j.issn.1671-0282.2018.07.011
Abstract
ObjectiveTo study the relationship between heparin-binding protein(HBP) and β2 integrin on the surface of neutrophil in acute lung injury.
MethodsA total of 30 mice from animal center of Tongji University were divided into control group, CLP group and antibody-treatment group by random number method. Acute lung injury animal model was established by cecal Ligation and puncture in the mice of CLP group and antibody-treatment group. Sham operations were performed on the mice in control group. Mice in antibody-treatment group received anti-CD18 antibody injected via tail vein 30 min before establishing acute lung injury animal model. Twenty-four hours after operation, mice were sacrificed and lung tissue was taken. After HE staining, lung injury were evaluated by Smith score. Lung tissue wet/dry weight ratio, BALF protein level, plasma HBP level and β2 integrin on neutrophil were measured. T test were used to compare the difference among groups. Pearson correlation was used to study the correlation between HBP, integrin and lung injury index.
ResultsCompared with control group, mice in CLP group had higher Smith score(t=10.607, P<0.01), lung wet/dry ratio(t=3.968, P=0.001),BALF protein level(t=4.331, P<0.01) and as well as higher plasma HBP(t=3.515, P=0.002) and β2 integrin(t=4.816, P<0.01) level. After CD18 antibody treatment, anti-treat group mice had lower Smith score (t=2.307, P=0.033), lung wet/dry ratio(t=3.080, P=0.006),BALF protein level(t=2.484, P=0.023) and as well as higher plasma HBP level(t=2.218, P=0.046) than mice in CLP group. Pearson correlation analysis showed HBP had obvious correlation with wet/dry ratio(r=0.527, P=0.017),BALF protein(r=0.508, P=0.022) and as well as β2 integrin(r=0.674, P=0.001).
ConclusionsBoth HBP and β2 integrin involved in the lung injury pathogenesis. HBP level is interrelated with the severity of lung injury. The β2 integrin is associated with the release of HBP by neutrophil. Inhabiting the function of integrin could decrease HBP level and alleviate the severity of lung injury.
Key words:
Acute respiratory distress syndrome; Acute lung injury; Heparin-binding protein; β2 integrin; Neutrophil
Contributor Information
Yang Liu
Intensive Care Unit, East Hospital, Tongji University School of Medicine, Shanghai 200120, China
Shaolin Ma
Intensive Care Unit, East Hospital, Tongji University School of Medicine, Shanghai 200120, China
Xuebin Wang
Intensive Care Unit, East Hospital, Tongji University School of Medicine, Shanghai 200120, China
Wei Xu
Intensive Care Unit, East Hospital, Tongji University School of Medicine, Shanghai 200120, China
Shouqin Zhang
Department of Critical Care, Tenth People’s Hospital of Tongji University, Shanghai 200072 , China
Xiangyu Zhang
Department of Critical Care, Tenth People’s Hospital of Tongji University, Shanghai 200072 , China
