Allergic Rhinitis
The comparation of establishing the allergic rhinitis model between Kunming mice and BALB/c mice
Gao Xian, Li Na, Zhang Jisheng, He Fan
Published 2017-07-07
Cite as Chin J Otorhinolaryngol Head Neck Surg, 2017,52(07): 501-505. DOI: 10.3760/cma.j.issn.1673-0860.2017.07.005
Abstract
ObjectiveTo discusses the feasibility of establishing the allergic rhinitis (AR) model in Kunming mice.
MethodsKunming mice (n=20) and BALB/c mice (n=20) were sensitized and motivated by ovalbumin (OVA) and aluminium hydroxide. The symptoms rating scale (symptoms score>5 as successful model) was used to evaluate AR symptoms of two kinds of mice after the AR model were set up. The differences of OVA serum specific IgE (OVA-sIgE) and the levels of interleukin 4 (IL-4) in Kunming mice model group and Kunming mice blank group were evaluated, the symptom scores, OVA serum specific IgE (OVA-sIgE) and the levels of interleukin 4 (IL-4) of successful model of Kunming mice and BALB/c mice were compared, and the difference between two kinds of animal models. SPSS 22.0 statistical software was used for statistical analysis was analysed. SPSS 22.0 statistical software was used for statistical analysis.
ResultsDuring the experiment, 2 mice died in BALB/c mouse model group, and no mouse died in Kunming mouse model group. Kunming model group gained a certain incease of weight. The clinical symptom scores, OVA - sIgE levels, serum IL - 4 levels of Kunming mouse model group were significantly higher than those of blank control group. The difference was statistically significant [(6.100±0.568) vs (2.700±0.823), (29.083±10.470) ng/ml vs (3.908±1.984) ng/ml, (219.250±30.821) pg/ml vs (140.056±27.684) pg/ml, all P<0.05]. Compared with BALB/c mice model group, the symptom score and serum level of IL-4 of Kunming mice model group had no statistically significant differences (6.100±0.568) vs (6.313±0.704), (219.250±30.821) pg/ml vs (253.294±53.953) pg/ml, all P>0.05), and the difference of serum level of OVA-sIgE was statistically significant ((29.083±10.470) ng/ml vs (76.277±25.724) ng/ml, P<0.05).
ConclusionsKunming mice are able to obtain a clinically significant AR model through OVA modeling, with a significant increase in serum OVA-SIgE and IL-4. Kunming mice AR model shows good tolerance, and could reduce the losses in animal experiment.
Key words:
Rhinitis, allergic; Models, animal; Mice; Feasibility studies
Contributor Information
Gao Xian
Department of Otorhinolaryngology Head and Neck Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266003, China (Now work at Department of Otorhinolaryngology Head and Neck Surgery, Qingdao Municipal Hospital)
Li Na
Department of Otorhinolaryngology Head and Neck Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266003, China
Zhang Jisheng
Department of Otorhinolaryngology Head and Neck Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266003, China
He Fan
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China
