Basic Researches
Changes of Th22 cells in rejection of the peripheral nerve xenotransplantation in mice
Huihui Chai, Liang Ma, Guizeng Chen, Shaopeng Li, Yanwu Guo, Bolin Wu, Yiquan Ke, Shizhong Zhang
Published 2015-01-15
Cite as Chin J Neuromed, 2015, 14(1): 9-13. DOI: 10.3760/cma.j.issn.1671-8925.2015.01.002
Abstract
ObjectiveTo evaluate the dynamic changes of Th22 cells in rejection of the peripheral nerve xenotransplantation in mice.
MethodsForty healthy male C57 BL/6 mice were randomly divided into isograft group and xenograft group (n=20). Catwalk automated gait analysis system was used to evaluate the motor function changes after isograft/xenograft treatment; immunohistochemical staining was used to observe to the interleukin (IL)-22 positive cell invasion degrees around the grafts at different time points. The percentages of Th22 cells in spleen were detected by flow cytometry; serum IL-22 levels were assessed by ELISA.
ResultsCatwalk-assisted gait analysis showed that xenograft group had significantly poorer motor and neurological functions as compared with isograft group (P<0.05). The degrees of IL-22+ cell invasion around the grafts in xenograft group significantly increased 5, 15, and 25 d after xenotransplantation as compared with those in the isograft group (P<0.05). As compared with those in the isograft roup ([5.10±0.28]%, [5.13±0.35]% and [4.92±0.34]%), markedly increased percentages of Th22 cells ([7.76±0.81]%, [9.6±0.73]% and [8.08±0.66]%) were noted in the xenograft group 5, 15, and 25 d after xenotransplantation (P<0.05). ELISA results revealed that the serum IL-22 levels during rejection in the xenograft recipients ([8.99±0.94] pg/m L, [11.0±1.08] pg/m L and [8.65±1.04] pg/mL) were statistically higher than those in the control group ([5.16±0.13] pg/mL, [5.22±0.22] pg/mL and [4.96±0.29] pg/mL) 5, 15, and 25 d after xenotransplantation (P<0.05).
ConclusionTh22 cells contribute to the rejection process of peripheral nerve xenotransplant.
Key words:
Xenotransplantation; Th22 cell; Interleukin-22; Rejection
Contributor Information
Huihui Chai
Department of Neurosurgery, Dongguan People's Hospital, Dongguan 523000, China
Liang Ma
Guizeng Chen
Shaopeng Li
Yanwu Guo
Bolin Wu
Yiquan Ke
Shizhong Zhang