Basic Research
Early administration of cyclosporin A providing neuroprotection in experimental rats with cerebral hemorrhage
Haiwang Zhang, Ling Zhang, Bin Wang, Hao Li, Changren Huang, Yang Ming, Jinghu Dong, Fubing Yang, Yong Jiang, Liang Liu, Xiangguo Xia, Hua Feng, Ligang Chen
Published 2015-11-15
Cite as Chin J Neuromed, 2015, 14(11): 1100-1105. DOI: 10.3760/cma.j.issn.1671-8925.2015.11.004
Abstract
ObjectiveTo assess the impact of early administration of cyclosporine A (CsA) in neuroprotection in experimental rats with intracerebral hemorrhage (ICH).
MethodsNinety-six male SD rats were randomly divided into sham-operated group, ICH+vehicle group, ICH+CsA 5 mg group and ICH+ CsA 10 mg group (n=24). And 100 mL autologous blood from the right femoral artery was injected into the right basal ganglia to induce ICH models in the later three groups. Tail vein administration of 5 mg/kg and 10 mg/kg CsA was performed in the later two groups 15 min after ICH, and then, once every 24 h. Twenty-four h after ICH, MR imaging was performed; brain water content was measured by Wet and dry weight method; blood-brain barrier permeability was detected by Evans blue (EB) method. Seventy-two h after ICH, NISSL and TUNEL were employed to detect the cell apoptosis, and neurological deficit scale scores were recorded.
ResultsTwenty-four h after ICH, ICH+ vehicle group had significantly severer cerebral edema, significantly increased water content of brain tissues (79.55%±0.09%) and EB content ([5.53±0.26] μg/g) as compared with sham-operated group (78.22%±0.14%, [2.55±0.13] μg/g), with significant differences (P<0.05); ICH+ CsA 10 mg group had significantly alleviated cerebral edema, significantly decreased water content of brain tissues (78.70%±0.07%) and EB content ([3.24±0.16] μg/g) as compared with ICH+vehicle group and ICH+ CsA 5 mg group (78.91%±0.11%, [4.12±0.12] μg/g), with significant differences (P<0.05). Seventy-two h after ICH, ICH+ vehicle group had decreased number of brain tissue cells around the hematoma, increased apoptosis cells (180.00±12.43) and decreased neurological deficit scale scores as compared with sham-operated group (26.00±7.29), with significant differences (P<0.05); ICH+CsA 10 mg group had decreased apoptosis cells (69.33±11.31) and increased neurological deficit scale scores as compared with ICH+vehicle group and ICH+CsA 5 mg group (95.00±8.99), with significant differences (P<0.05).
ConclusionEarly application of CsA can improve brain edema, blood-brain barrier permeability, decrease neuronal apoptosis and improve neurological deficit scale scores after ICH.
Key words:
Intracerebral hemorrhage; Cyclosporin A; Brain edema; Blood brain barrier; Neuronal apoptosis
Contributor Information
Haiwang Zhang
Department of Neurosurgery, Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
Ling Zhang
Department of Neurosurgery, Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
Bin Wang
Department of Neurosurgery, Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
Hao Li
Department of Neurosurgery, Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
Changren Huang
Department of Neurosurgery, Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
Yang Ming
Department of Neurosurgery, Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
Jinghu Dong
Department of Neurosurgery, Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
Fubing Yang
Department of Neurosurgery, Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
Yong Jiang
Department of Neurosurgery, Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
Liang Liu
Department of Neurosurgery, Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
Xiangguo Xia
Department of Neurosurgery, Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
Hua Feng
Ligang Chen
Department of Neurosurgery, Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
