Basic Research
Neuroprotective effect of 2-methoxyestradiol on neurological function and nerve cell apoptosis in rats with acute cerebral infarction
Peng Jinghua, Jin Fei, Zhang Hongri, Zhang Yude
Published 2017-02-15
Cite as Chin J Neuromed, 2017,16(02): 139-142. DOI: 10.3760/cma.j.issn.1671-8925.2017.02.006
Abstract
ObjectiveTo investigate the neuroprotective effect of 2-methoxyestradiol (2ME2) on neurological functions and nerve cell apoptosis in rats with acute cerebral infarction.
MethodsSeventy-two adult male SD rats were randomly divided into sham-operated group, model group and treatment group (n=24). Middle cerebral artery infarction models in the later two groups were constructed by Longa method. Rats in treatment group were treated with 2ME2 (24 mg/kg) daily for 7 d, while rats in the sham-operated group and model group with physiological saline. Bederson standard scale was adopted to analyze the nerve function defect 7 d after operation. Caspase-3 protein expression in the rat brain tissues was analyzed by Western blotting and apoptosis index was analyzed by TUNEL.
ResultsAs compared with those in the model group (2.015±0.871), Bederson standard scale scores in the treatment group (1.235±0.849) were significantly lower (P<0.05); as compared with the sham-operated group (0.286±0.160, 7.8±6.9), the model group and treatment group had significantly increased Caspase-3 protein level and apoptosis index (P<0.05), while Caspase-3 protein level and apoptosis index in treatment group (0.615±0.185, 27.8±6.4) were significantly lower than those in the model group (1.109±0.224, 34.9±5.9, P<0.05).
Conclusion2ME2 can improve the neurological function, reduce the level of apoptosis in rat brain nerve cells and has a protective effect on nerve.
Key words:
2-methoxyestradiol; Cerebral infarction; Apoptosis; Neurological function
Contributor Information
Peng Jinghua
Neurology Department, First Affiliated Hospital, Henan University of Science and Technology, Luoyang 471003, China
Jin Fei
Department of First Pharmacy, First Affiliated Hospital, Henan University of Science and Technology, Luoyang 471003, China
Zhang Hongri
Neurosurgery Department, First Affiliated Hospital, Henan University of Science and Technology, Luoyang 471003, China
Zhang Yude
Neurology Department, First Affiliated Hospital, Henan University of Science and Technology, Luoyang 471003, China