Basic Research
Early diagnosis of Parkinson's disease by PET/CT apoptosis imaging with tracer of 18F-ML-10
Shiming Huang, Wei Chen, Jinming Zhang, Liang Yin, Jianlan Yue, Yanfeng Li, Wenjie Ji, Xin Zhou, Yongqiang Ma, Huipeng Meng, Zhichun Lin
Published 2017-05-15
Cite as Chin J Neuromed, 2017, 16(5): 452-457. DOI: 10.3760/cma.j.issn.1671-8925.2017.05.004
Abstract
ObjectiveThe 18F-ML-10 PET/CT apoptosis imaging was taken in the early Parkinson's disease (PD) rat models. The feasibility of diagnosis of PD with 18F-ML-10 PET/CT apoptosis imaging is explored.
MethodsTwenty adult healthy male Sprague-Dawley rats were randomly divided into control group and PD model group (n=10). Intrastriat administration of 6-hydroxy dopamine (6-OHDA) was performed to induce progressive and retrograde degenerative changes in the substantial nigra of neurons (PD models). One week after apomorphine inducement, the rotational behaviors of the rats were evaluated. 11C-CFT PET/CT imaging and 18F-ML-10 PET/CT were performed to observe the dopamine transport protein expression in the corpus striatum and apoptosis of dopaminergic neurons in the substantia nigra. Immunofluorescence staining of anti-tyrosine hydroxylase (TH) antibody was performed to evaluate the survival of dopaminergic cells in the compact part of substantia nigra. TUNEL was performed to evaluate the apoptosis of dopaminergic cells in the compact part of substantia nigra. Nissl staining was performed to detect the cellular morphology.
ResultsOne week after PD modeling, the rats in the experimental group obviously rotated to the contralateral, and the average rotation speed was (4.52±1.03) r/min. The ratio of 11C-CFT between the right and left striatum of rats in the experimental group was 0.556±0.017, which was significantly lower than that of the control group (0.998±0.013, P<0.05). The radioactivity ratio of 18F-ML-10 between the right and left substantia nigra of rats was 1.722±0.083, which was significantly higher than that of the control group (1.024±0.056, P< 0.05). Immunofluorescence showed that the ratio of TH-positive neurons between the right and left compact part of substantia nigra in rats of the experimental group was 0.528±0.012, which was significantly lower than that of the control group (1.036±0.030, P<0.05). TUNEL showed that the number of dopaminergic neuronal apoptosis in the experimental group was 43.200±2.507, which was significantly larger than that of the control group (1.400±0.427, P<0.05).
ConclusionPD is associated with apoptosis of dopaminergic neurons; 18F-ML-10 PET/CT imaging can be used to diagnose PD in the early-stage.
Key words:
18F-ML-10; PET/CT; Parkinson's disease; Dopaminergic neuron; Apoptosis
Contributor Information
Shiming Huang
Department of Nuclear Medicine, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China
Wei Chen
Department of Nuclear Medicine, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China
Jinming Zhang
Department of Nuclear Medicine, PLA General Hospital, Beijing 100853, China
Liang Yin
Department of Nuclear Medicine, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China
Jianlan Yue
Department of Nuclear Medicine, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China
Yanfeng Li
Department of Nuclear Medicine, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China
Wenjie Ji
Key Laboratory Remodeling and Target Organ Damage, Tianjin 300162, China
Xin Zhou
Key Laboratory Remodeling and Target Organ Damage, Tianjin 300162, China
Yongqiang Ma
Key Laboratory Remodeling and Target Organ Damage, Tianjin 300162, China
Huipeng Meng
Department of Oncology, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China
Zhichun Lin
Department of Nuclear Medicine, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China
