Original Article
Molecular epidemiological investigation of an intra-familial transmission of HIV-1 CRF02_AG
Xin Ruolei, Liu An, Sun Lijun, Li Jia, Zhang Qin, Lu Hongyan, Sun Weidong
Published 2019-02-25
Cite as Int J Virol, 2019,26(1): 21-25. DOI: 10.3760/cma.j.issn.1673-4092.2019.01.06
Abstract
ObjectiveTo investigate an intra-familial transmission event of human immunodeficiency virus type 1 (HIV-1) using molecular epidemiological method, and to elucidate the spreading route among the family members.
MethodsEpidemiological investigation, including follow-up and testing, was conducted for a pregnant woman who was anti-HIV screening-reactive, her husband and their newborn. Viral RNA was extracted to amplify HIV-1 gag gene and pol gene segments with one-step RT-PCR. Phylogenetic tree was constructed to analyze the spreading route.
ResultsThe proband (EID1107M) was a pregnant woman who was found to be HIV-seropositive during pregnant care. Her husband (EID1107F) was then confirmed to be anti-HIV positive. Her newborn (EID1107I) was positive for HIV-1 nucleic acid at age of eight months. All three patients were infected with HIV-1 CRF02_AG strain. Phylogenetic tree showed that the three patients formed a subcluster with high genetic homogeneity. The topological structure indicated the transmission route was from the father to the mother, then to their child.
ConclusionContagious diseases screening should be reinforced before marriage and during pregnant care. Prevention of mother-to-child transmission should be timely and efficiently administered upon diagnosis of infection, to avoid the secondary intra-familial transmission and to improve the population quality at birth.
Key words:
Human immunodeficiency virus type 1; Intra-familial; Mother-to-child transmission; Molecular epidemiology
Contributor Information
Xin Ruolei
Institute of STD/AIDS Prevention and Control, Beijing Municipal Center for Disease Prevention and Control, Beijing Center for Preventive Medicine Research, Beijing 100013, China
Liu An
Center for STD/AIDS Diagnosis and Therapy, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China
Sun Lijun
Center for STD/AIDS Diagnosis and Therapy, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China
Li Jia
Institute of STD/AIDS Prevention and Control, Beijing Municipal Center for Disease Prevention and Control, Beijing Center for Preventive Medicine Research, Beijing 100013, China
Zhang Qin
Institute of STD/AIDS Prevention and Control, Beijing Municipal Center for Disease Prevention and Control, Beijing Center for Preventive Medicine Research, Beijing 100013, China
Lu Hongyan
Institute of STD/AIDS Prevention and Control, Beijing Municipal Center for Disease Prevention and Control, Beijing Center for Preventive Medicine Research, Beijing 100013, China
Sun Weidong
Institute of STD/AIDS Prevention and Control, Beijing Municipal Center for Disease Prevention and Control, Beijing Center for Preventive Medicine Research, Beijing 100013, China