Basic Research
Virus isolation and gene evolution analysis on Jiangsu local strain of coxsackievirus B4 type
Xia Zhiqiang, Song Qinqin, Song Juan, Xue Qingkai, Wang Yanhai, Han Jun
Published 2020-02-25
Cite as Int J Virol, 2020,27(01): 32-35. DOI: 10.3760/cma.j.issn.1673-4092.2020.01.008
Abstract
ObjectiveTo established the isolation method of coxsackievirus B4 (CVB4), and to perform genetic evolution analysis of the isolated Jiangsu local strain.
MethodsFluorescence quantitative PCR was used to detect enteroviruses in the throat swabs from patients with respiratory infections admitted to the sentinel hospitals of Jiangsu province. The positive samples were used for virus isolation with HeLa cell line, followed by VP4 gene sequencing and phylogenetic analysis.
ResultsTwelve samples were positive of enterovirus by fluorescence quantitative PCR. Cytopathic effect was observed in one sample during virus isolation. The VP4 gene were amplified by specific RT-PCR. The sequence analysis showed that the similarities of the VP4 gene sequence with the other 20 CVB4 strains ranged from 86.56% to 93.59%, and the strain was identified as a Jiangsu local strain of CVB4. The phylogenetic tree showed that the strain only clustered with CVB4 strain KY369904.
ConclusionsHeLa cell line was used to isolate and identify one strain of enterovirus in the present study. The virus strain was identified as Jiangsu local strain of CVB4 using evolution analysis of VP4 gene. The work presented important value for the prevention and control of local enterovirus infections.
Key words:
Coxsackievirus B4; Enterovirus; Virus isolation; Cytopathic effect
Contributor Information
Xia Zhiqiang
State Key Laboratory of Infectious Disease Prevention and Control, National Institute of Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Song Qinqin
State Key Laboratory of Infectious Disease Prevention and Control, National Institute of Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Song Juan
State Key Laboratory of Infectious Disease Prevention and Control, National Institute of Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Xue Qingkai
School of Public Health, Wannan Medical College, Wuhu 241000, China
Wang Yanhai
State Key Laboratory of Infectious Disease Prevention and Control, National Institute of Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Han Jun
State Key Laboratory of Infectious Disease Prevention and Control, National Institute of Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
