SFRP2 gene promoter hypermethylation and its clinicopathologic significance in colorectal carcinoma
YU Hai-feng, WANG Dao-rong, ZHANG Yun, JIANG Chao, ZHOU Su-jun, ZHANG Guo-qiang, LI Yong-kun, CHEN Jie, TANG Dong, CHA Tian-zhou
Published 2012-08-15
Cite as , 2012,39(08): 526-529,封3. DOI: 10.3760/cma.j.issn.1673-4203.2012.08.007
Abstract
Objective To explore the differential level of hypermethylated SFRP2 gene in colorectal cancers andadjacent nontumorous tissues,to analyze the relation of SFRP2 gene promoter hypermethylation status and its clinicopathologic significance in colorectal carcinoma,and to study the relationship between the level of hypermethylated SFRP2 and the invasion and metastasis of colorectal carcinoma.Methods Real-time quantitative PCR technique was performed to analyze the level of hypermethylated SFRP2 gene promoter in colorectal cancers and adjacent nontumorous tissues taken from 30 colorectal cancer patients.The relation of the level of hypermethylated SFRP2 gene promoter and its clinicopathologic features of colorectal cancers was analyzed.Results SFRP2 gene promoter hypermethylation occurred in both the tumor tissues and the adjacent tissues.The level of SFRP2 gene promoter hypermethylation was significantly higher in the patients with TNM Ⅲ and Ⅳ (7.24 ± 1.13)than in patients with TNM Ⅰ and Ⅱ ( 5.92 ± 0.97 ) ( P < 0.05 ).The level of SFRP2 gene promoter hypermethylation was significantly higher in the patients with lower differentiation(7.31 ± 1.11 ) than in patients with higher differentiation (6.23 ± 1.03) ( P < 0.05 ).No significant association was found between the level of SFRP2 gene promoter hypermethylation and the status of gender,age.tumor location and tumor size ( P > 0.05 ).Conclusions SFRP2 gene hypermethylation in tissues may be a useful objective parameter for the malignant level,invasion,metastasis,recurrence and prognosis of the colorectal cancer.
Key words:
Colorectal neoplasms; Tumor-infiltrating; Neoplasm metastasis; Methylation; Polymerase chain reaction; Pathology, clinical
Contributor Information
YU Hai-feng
Department of General Surgery, Yixing People's Hospital, Jiangsu University, Yixing 214200, China
WANG Dao-rong
Department of Gastrointestinal Surgery, Northern Jiangsu People's Hospital, Yangzhou225001, China
ZHANG Yun
Department of General Surgery, Yixing People's Hospital, Jiangsu University, Yixing 214200, China
JIANG Chao
Department of General Surgery, Yixing People's Hospital, Jiangsu University, Yixing 214200, China
ZHOU Su-jun
Department of General Surgery, Yixing People's Hospital, Jiangsu University, Yixing 214200, China
ZHANG Guo-qiang
Department of General Surgery, Yixing People's Hospital, Jiangsu University, Yixing 214200, China
LI Yong-kun
Department of Gastrointestinal Surgery, Northern Jiangsu People's Hospital, Yangzhou225001, China
CHEN Jie
Department of Gastrointestinal Surgery, Northern Jiangsu People's Hospital, Yangzhou225001, China
TANG Dong
Department of Gastrointestinal Surgery, Northern Jiangsu People's Hospital, Yangzhou225001, China
CHA Tian-zhou
Department of General Surgery, Yixing People's Hospital, Jiangsu University, Yixing 214200, China