Experimental and Prescriptions of Chinese Medicine
Simultaneous determination of isoquercetin and astragalin in Moringa oleifera leaves prescription-a prescription for treatment of constipation by HPLC
Liu Aoxue, Gu Wenhong, Li Mengwei, Chen Xinjing, He Jun, Long Xingfen, Liu Yang
Published 2018-02-28
Cite as Int J Trad Chin Med, 2018,40(2): 153-156. DOI: 10.3760/cma.j.issn.1673-4246.2018.02.014
Abstract
ObjectiveTo establish an HPLC method for simultaneous determination of Isoquercitrin and astragalin in Moringa oleifera leaves prescription.
MethodsBy using HPLC method with Cosmosil-C18 column (4.6 mm × 250 mm, 5 μm) and the column temperature was 40 degrees Celsius, the mobile phase contains a 0.1% phosphoric acid in water(A) and acetonitrile(B), the detection wavelength was set at 350nm by UV detector and the flow rate was 1.3 ml/min.
ResultsThe Isoquercitrin and astragalin was better separated in 30 minutes, and Isoquercitrin had a good linear correlation at the range of 0.24-4.73 μg, while astragalin was 0.11-2.19 μg, the average spiked recoveries of isoquercetin and astragalin were 99.09% (RSD=0.60%) and 99.08% (RSD=1.37%), respectively.
ConclusionsThe proposed method is simple,accurate,and repeatable and can be used for quality control as well as a powerful tool to evaluate the processing of Moringa oleifera leaves. It can provide the reference basis and data support for the determination of content of Moringa oleifera health products.
Key words:
Chromatography, high pressure liquid; Chemical quantitative analysis; Moringa oleifera; Relaxing bowels compounds
Contributor Information
Liu Aoxue
Master of Grade 2015, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
Gu Wenhong
Li Mengwei
Chen Xinjing
He Jun
Long Xingfen
Liu Yang
