Experimental and Prescriptions of Chinese Medicine
Protective effects of curcumin on oxidative stress injury of H9C2 cardiac myocytes induced by H2O2
Longmei Wu, Chunyan Liu
Published 2019-01-30
Cite as Int J Trad Chin Med, 2019, 41(1): 57-61. DOI: 10.3760/cma.j.issn.1673-4246.2019.01.014
Abstract
ObjectiveTo study the protective effects of curcumin on oxidative stress injury of H9C2 cardiac myocytes induced by H2O2.
MethodsThe oxidative stress injury model in H9C2 cardiac myocytes was established in vitro, and cells were divided into the control group, H2O2 group (200 μmol/L), low- (10 μmol/L), medium- (20 μmol/L), high- (40 μmol/L) dose curcumin, and each group set six holes. After H2O2 stimulation for 24 h, the morphology changes were observed by microscope, and the survival rates were measured using CCK8 method. The activity of LDH, CK, and AST, and the content of MDA in culture medium were detected, and the activities of SOD, CAT, and GSH-Px in cardiac myocytes were also determined.
ResultsCompared with the H2O2 group, the activity of LDH (247.36 ± 28.04 U/L, 195.14 ± 21.37 U/L, 132.27 ± 16.33 U/L vs. 247.58± 28.34 U/L), AST (67.27 ± 10.58 U/ml, 54.81 ± 8.60 U/ml, 42.14 ± 5.95 U/ml vs. 84.34 ± 12.36 U/ml), CK (1.34 ± 0.66 U/ml, 0.95 ± 0.42 U/ml, 0.71 ± 0.39 U/ml vs. 1.56 ± 0.74 U/ml) and the content of MDA (227.39 ± 32.05 nmol/ml, 185.11 ± 24.37 nmol/ml, 143.50 ± 16.37 nmol/ml vs. 274.19 ± 36.51 nmol/ml) in culture medium of curcumin were significantly decreased (P<0.05). The morphology of H9C2 cardiac myocytes in curcumin group was improved, the survival rate was significantly increased (P<0.05). The activity of SOD (17.67± 1.36 U/mg, 21.54 ± 1.72 U/mg, 28.37 ± 2.36 U/mg vs. 14.37 ± 1.22 U/mg), CAT (19.58 ± 3.87 U/mg, 25.34 ± 4.06 U/mg, 30.21 ± 4.83 U/mg vs. 14.77 ± 3.25 U/mg) and GSH-Px (1.99 ± 1.17 U/mg, 2.56 ± 1.29 U/mg, 3.04 ± 0.45 U/mg vs. 1.67 ± 0.87 U/mg) in cardiac myocytes were significantly increased (P<0.05).
ConclusionsCurcumin can effectively improve oxidative stress injury of H9C2 cardiac myocytes induced by H2O2, and curcumin has dose-dependent protective effects against the oxidative stress of H9C2 cardiac myocytes induced by H2O2.
Key words:
Curcumin; H9C2 cardiac myocytes; Hydrogen peroxide; Oxidative stress; Cell proliferation
Contributor Information
Longmei Wu
Cardiovascular Department, National Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi 445000, China
Chunyan Liu
Department of Endocrinology, Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi 445000, China
