Objective To investigate the metabolic characteristics of early stage of obstructive chronic pancreatitis (CP) in swine by high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy.Methods A total of 39 Bama miniature swine were randomly divided into CP group and control group.The pancreatic main duct (PMD) of the swine in the experimental group underwent partial proximal ligation procedure.The swine in the control group underwent opening the abdomen and rubbing the pancreas and intestine.At the 4th,8th and 12th week after the operation,the swine were sacrificed to harvest the pancreas tissue for pathologically examination,which were classified as mild,moderate and severe according to the degree of fibrosis.The metabolic profiles from the pancreas of normal swine and mild CP swine were examined by using HR-MAS NMR spectroscopy.Results CP model was successfully established in 24 Bama miniature swine,and pathologically examination showed 10 was mild,5 was moderate and 7 was severe.Among the metabolites in mild CP,HR-MAS NMR analysis suggested that the peak of choline,glycine,betaine,lactate,fatty acid,phosphorylcholine/choline glycerophosphate was different with those in normal pancreas,and only the difference of choline was statistically significant (5.02 ± 1.96 vs 1.56 ± 0.79,P ＜ 0.O1),while the difference of other 5 metabolites was not statistically significant.Conclusions Compared with control group,mild CP in Bama miniature swine shows significantly increased choline,and this may be a marker for discrimination of normal and early CP.