Biotechnology
Mechanism underlying the regulation of microRNA-223-3p on proliferation and invasion of thyroid cancer cells via targeting MTSS1 gene
Shi Pei, Shen Huwei, Liu Haihua
Published 2019-06-15
Cite as Chin J Biomed Eng, 2019,25(3): 293-299. DOI: 10.3760/cma.j.issn.1674-1927.2019.03.007
Abstract
ObjectiveTo investigate the effects of microRNA (miR) -223-3p on proliferation and invasion of thyroid cancer cells and the potential mechanism.
MethodsReal-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression levels of miR-223-3p and MTSS1 in thyroid cancer SW579 cells and normal thyroid Nthy-ori 3-1 cell-line. The SW579 cells were subject to different protocol of liposome transfection and divided into miR-NC group (transfected with miR-NC) , miR-223-3p group (transfected with miR-223-3 mimics) , pcDNA group (transfected with pcDNA) , pcDNA-MTSS1 group (transfected with pcDNA-MTSS1) , anti-miR-NC group (transfected with anti-miR-NC) , anti-miR-223-3p group (transfected with anti-miR-223-3p) , miR-NC + WT-MTSS1 group (co-transfected with miR-NC and WT-MTSS1) , miR-NC+MUT-MTSS1 group (co-transfected with miR-NC and MUT-MTSS1) , miR-223-3p+ WT-MTSS1 group (co-transfection with miR-223-3p and WT-MTSS1) , miR-223-3p+MUT-MTSS1 (co-transfected with miR-223-3p and MUT-MTSS1) , anti-miR-223-3p + si-NC group (co-transfected with anti-miR-223-3p and si-NC) and anti-miR-223-3p+si-MTSS1 (co-transfected with anti-miR-223-3p and si-MTSS1) . For each group, cell proliferation was detected by CCK-8 assay, cell aptopsis by flow cytometry, MTSS1 protein expression by Western blotting, and fluorescence cell viability by dual luciferase reporter gene assay.
ResultsCompared with normal thyroid Nthy-ori 3-1 cells, the thyroid cancer SW579 cells showed significantly increased expression level of miR-223-3p and decreased expression levels of MTSS1 mRNA and protein (P<0.05) . miR-223-3p may negatively target-regulate the MTSS1 expression. Either suppressed expression of miR-223-3p or overexpression of MTSS1 may inhibit the proliferation and promote apoptosis of SW579 cells. Suppressed MTSS1 expression may regress the inhibitory effect of miR-223-3p down-regulation on proliferation and apoptosis of thyroid cancer SW578 cells.
ConclusionmiR-223-3p may inhibit the proliferation and promote apoptosis of thyroid cancer SW578 cells. The mechanism may be related to its targeted regulation of MTSS1, which reveals a new target for prevention and treatment of thyroid cancers.
Key words:
miR-223-3p; MTSS1; Thyroid cancer; Proliferation; Apoptosis
Contributor Information
Shi Pei
Department of Endocrinology, Heping Hospital Affiliated to Changzhi Medical College, Changzhi, Shanxi 046000, China
Shen Huwei
Liu Haihua
