Objective To study the mRNA and protein expression of sal-like gene 4 (SALL4) in children's acute leukemia (AL).Methods Through the real-time semi-quantitative reserve transcriptase-polymerase chain reaction(RT-PCR) method to test the mRNA expression level of SALL4 in the bone marrow of children' s AL and normal control group,explore the protein expression level of SALL4 in enzyme-linked immuno sorbent assay(ELISA),and proceed the correlation analysis.Results ①There was a statistically significant expression between AL and control group (P ＜ 0.05),the mRNA expression levels of SALL4 in ALL and ANLL were significantly higher than those in control group (P ＜ 0.05),there was a statistically significant expression between B-ALL and T-ALL (P ＜ 0.05),but the mRNA expression level of SALL4 has no difference between B-ALL and ANLL(P ＞ 0.05).②The amount of SALL4 protein expression in B-ALL,T-ALL,ANLL was respectively 136 ± 17,33 ±23,151 ±64,55 ±16.③ The mRNA and protein expression of SALL4 was positively correlated(r =0.931,P ＜0.01).Conclusions There is abnormal higher expression of SALL4 in children' s AL bone marrow mononuclear cells,it is statistically significant compared with the normal control group,and suggest that SALL4 is involved in the pathogenesis of children' s AL.In children' s AL,the mRNA and protein expression of SALL4 is higher in B-ALL and ANLL,and positively related,but they don't express in T-ALL and the normal control group.It suggests that the pathogenesis in children' s B-ALL and T-ALL may be different,and the pathogenesis in children's B-ALL and ANLL may be same.