Objective To investigate the clinical value of combined detection of CEA,TSGF,OPN and CA125 both in nipple discharge and serum for the diagnosis of breast cancer and intraductal proliferative lesions.Methods The serum and nipple discharge of 122 patients(21 cases of breast cancer and 101 cases of benign lesions) was collected and the tumor markers of CEA,TSGF,OPN and CA125 were detected by electrochemiluminescence method,and those were compared with those of 30 healthy pregnant women.And the relationship between nipple discharge and serum tumor markers and breast benign and malignant disease was analyzed.Results The nipple discharge and serum levels of CEA,TSGF,OPN and CA125 in bresat cancer patients were significantly higher than those in the patients with benign breast disease and those in normal pregnant women(P ＜0.01).The levels of CA153,TSGF,CA125 and CEA in nipple discharge were significantly higher than in those in the serum(P ＜0.01).The sensitivity of the dyn amic combined detection of the four tumor markes in nipple discharge and serum was 95.24％.ConcluSions The levels of CEA,TSGF,OPN and CA125 in nipple discharge were significantly higher than those in the serum.The dynamic combined detection of the four tumor markers both in nipple discharge and serum are benefit for early diagnosis and interference and better prewarning markers for monitoring the recurrence and metastasis of breast cancer.