Original Article
Establishment of a method to detect the level of saliva 1,5-anhydroglucitol
Jian Chaohui, Zhao Aihua, Xu Yiting, Wang Yansu, Nie Xiaomin, Ying Lingwen, Lu Wei, Zhu Wei, Ma Xiaojing, Zhou Jian, Bao Yuqian
Published 2020-01-27
Cite as Chin J Diabetes Mellitus, 2020,12(01): 25-29. DOI: 10.3760/cma.j.issn.1674-5809.2020.01.005
Abstract
ObjectiveTo establish a method to detect the level of saliva 1,5-anhydroglucitol (1,5-AG).
MethodsFrom June 2017 to March 2018, 100 subjects (50 men and 50 women) with normal glucose tolerance (NGT group, n=69) or diabetes mellitus (DM group, n=31) were enrolled in the Sixth People′s Hospital affiliated to Shanghai Jiao Tong University. All subjects chewed swabs in Salivette saliva collection tubes to collect saliva. Enzymatic assay was used to explore the collection and storage methods for saliva 1,5-AG measurements. The mass spectrometry method was explored to analyze the saliva 1,5-AG levels. Differences were compared between NGT subjects and DM patients. Methods of t test and Mann-Whitney U test were used for comparison between groups, and the Spearman correlation was used for correlation analysis.
Results(1) Brushing teeth before saliva collection had no effect on saliva 1,5-AG levels. There was no significant difference in the volume of saliva between chewing 40-50 times, 50-60 times, and 60-70 times per minute (all P>0.05). The 1,5-AG levels in saliva stored at room temperature or 4 ℃ for 2 hours were not different from those detected immediately after collection (both P>0.05).(2) Compared with NGT subjects, DM patients had lower saliva 1,5-AG levels [(1.17±0.24) vs (0.94±0.19) μg/ml, t=4.084] and serum 1,5-AG levels [22.0 (17.6, 28.5) vs 4.6 (2.8, 8.2) μg/ml, Z=-5.421] (both P<0.05). The saliva 1,5-AG levels were positively correlated with serum 1,5-AG levels (r=0.528, P<0.05) and negatively correlated with 2 hour plasma glucose, glycated hemoglobin and glycated albumin (r=-0.366, -0.543, -0.444, respectively; all P<0.05).
ConclusionsSaliva collection is simple and the sample can be stored at room temperature or 4 ℃ for a short time. Saliva 1,5-AG levels can significantly differentiate DM patients from NGT subjects, which is expected to be a supplementary indicator for evaluating short-term glycemic control in DM patients.
Key words:
Diabetes mellitus; Mass spectrometry analysis; Saliva collection; 1,5-Anhydroglucitol
Contributor Information
Jian Chaohui
Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People′s Hospital, Shanghai Clinical Center for Diabetes, Shanghai 200233, China
Zhao Aihua
Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People′s Hospital, Shanghai 200233, China
Xu Yiting
Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People′s Hospital, Shanghai Clinical Center for Diabetes, Shanghai 200233, China
Wang Yansu
Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People′s Hospital, Shanghai Clinical Center for Diabetes, Shanghai 200233, China
Nie Xiaomin
Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People′s Hospital, Shanghai Clinical Center for Diabetes, Shanghai 200233, China
Ying Lingwen
Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People′s Hospital, Shanghai Clinical Center for Diabetes, Shanghai 200233, China
Lu Wei
Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People′s Hospital, Shanghai Clinical Center for Diabetes, Shanghai 200233, China
Zhu Wei
Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People′s Hospital, Shanghai Clinical Center for Diabetes, Shanghai 200233, China
Ma Xiaojing
Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People′s Hospital, Shanghai Clinical Center for Diabetes, Shanghai 200233, China
Zhou Jian
Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People′s Hospital, Shanghai Clinical Center for Diabetes, Shanghai 200233, China
Bao Yuqian
Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People′s Hospital, Shanghai Clinical Center for Diabetes, Shanghai 200233, China
