Specific Original Articles on Thyroid and Parathyroid Diseases
The clinical significance and biological function of microRNA-106b in thyroid cancer
Yuanmei Li, Limin Sun, Yuanyuan Wang, Boning Cheng
Published 2017-02-25
Cite as Chin J Endocr Surg, 2017, 11(1): 60-64. DOI: 10.3760/cma.j.issn.1674-6090.2017.01.014
Abstract
ObjectiveTo investigate clinical significance and function of microRNA-106b (miR-106b) in retinoblastoma tissues and cells.
MethodsWe detected miR-106b expression in 51 samples of thyroid cancer and the adjacent non-tumor tissues using qRT-PCR. The expression of miR-106b was altered by corresponding vectors in thyroid cancer cells, and then BrdU cell proliferation and flow cytometry assay were performed to examine the proliferation and apoptosis of thyroid cancer cells.
ResultsThe expression of miR-106b in thyroid cancer tissues was significantly lower than that in normal tumor-adjacent tissues (0.36±0.029 vs 0.98±0.034, P=0.004) . MiR-106b expression in tumor tissues was significantly associated with tumor size and tumor number. MiR-106b was obviously inhibited by miR-106b inhibitor in PTC-1 cells (0.96±0.025 vs 0.29±0.032, P=0.001) , and inhibition of miR-106b resulted in significantly increased proliferation (89.33±5.67 vs 136.67±10.33, P=0.03) and decreased apoptosis (16.33±3.20 vs 7.67±2.45, P=0.04) . On the contrast, over-expression of miR-106b using miR-106b mimics led to significantly decreased proliferation (98.00±4.65 vs 76.33±2.87, P=0.03) and increased apoptosis (22.54±2.13 vs 32.45±4.34, P=0.04) .
ConclusionsDecreased expression of miR-106b is correlated with the adverse clinicopathological features of thyroid cancer. MiR-106b can inhibit cell proliferation and apoptosis of thyroid cancer cells, suggesting miR-106b plays a suppressive role in development and progression of thyroid cancer.
Key words:
MicroRNA-106b; Thyroid cancer; Apoptosis; Proliferation
Contributor Information
Yuanmei Li
Department of Endocrinology, the Sixth People’s Hospital of Hangzhou, Hangzhou 310000, China
Limin Sun
Department of Pathology, the Third Affiliated Hospital of Wenzhou Medical University, Wenzhou 325200, China
Yuanyuan Wang
Department of Endocrinology, the Sixth People’s Hospital of Hangzhou, Hangzhou 310000, China
Boning Cheng
Department of Endocrinology, the Sixth People’s Hospital of Hangzhou, Hangzhou 310000, China