Original Article
The expression and role of Caspase3, Caspase12 and CHOP in pituitary tissues of rats with acute necrotizing pancreatitis
Wenhong Deng, Wenyi Guo, Xiaobo He, Fangchao Mei, Yupu Hong, Xiaojia Yang, Weixing Wang
Published 2018-12-25
Cite as Chin J Endocr Surg, 2018, 12(6): 478-482. DOI: 10.3760/cma.j.issn.1674-6090.2018.06.009
Abstract
ObjectiveTo observe the pathological changes of pituitary tissue in rats with acute necrotizing pancreatitis and to explore the mechanism of pituitary tissue injury in rats.
Methods24 SD rats were randomly divided into normal group (N group, n=8) , sham operation group (SO group, n=8) , and acute necrotizing pancreatitis group (ANP group, n=8) . ANP model was established by retrograde injection of 5% sodium taurocholate into the biliopancretic duct. The serum levels of amylase (AMY) and lipase (LIP) were detected by automatic biochemical. The serum levels of growth hormone (GH) , adrenocorticotropic hormone (ACTH) , thyroid stimulating hormone (TSH) and follicle-stimulating hormone (FSH) were measured by radioimmunoassay. The pathological changes of pancreatic tissue and pituitary tissue were observed by the light microscope. The expression of Casepase3, Caspase12 and CHOP in pituitary tissue were determined by immunohistochemical method.
ResultsCompared to SO group, the serum levels of AMY (8679.16±307.60) U/L and LIP (9376.83±380.92) U/L were significantly higher in ANP group (P<0.05) . The serum levels of ACTH (0.92±0.41) pg/ml, TSH (0.14±0.06) pg/ml, and FSH (2.01±0.38) pg/ml were significantly lower in ANP group (P<0.05) . The expression of Caspse 3 (65.66±7.58) , Caspase12 (70.66±4.76) and CHOP (143.16±19.05) in pituitary tissue were significantly increased in ANP group (P<0.05) . The pancreatic injury was more severe in ANP group under light microscope (P<0.05) . The degree of hyperemia of pituitary tissue of ANP group was aggravated.
ConclusionPathological changes occur in rat pituitary tissues and endoplasmic reticulum stress injury plays a role in pituitary injury during ANP.
Key words:
Acute necrosis pancreatitis; Pituitary; Apoptosis; Caspase3; Caspase12; CHOP
Contributor Information
Wenhong Deng
Department of General Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China
Wenyi Guo
Xiaobo He
Fangchao Mei
Yupu Hong
Xiaojia Yang
Weixing Wang