Original Article
miR-9 on the proliferation of breast cancer cells by targeting hexokinase 2
Wang Yuanbin, Zhao Yaxin, Yang Lijun, Zheng Jinying, Tang Jundong, Ma Ruiming
Published 2020-02-25
Cite as Chin J Endocr Surg, 2020,14(01): 52-55. DOI: 10.3760/cma.j.issn.1674-6090.2020.01.012
Abstract
ObjectiveTo study the regulatory effect of miR-9 on the proliferation of breast cancer cells by targeting hexokinase 2 (HK2) .
MethodsBreast cancer tissues and paracancer tissues were collected and the expression levels of miR-9 and HK2 were detected. MCF-7 cells were cultured and divided into blank control group, NC group, miR-9 group, NC-siRNA group and HK2-siRNA group. The cell viability, expression levels of HK2 and cleaved caspase-3 were detected, the targeted binding of miR-9 to HK2 was verifed.
Resultsthe expression level of miR-9 in breast cancer was lower than that in paracancer tissue (0.52±0.08 vs 1.05±0.25, t=16.685, P<0.000) , and the expression level of HK2 was higher than that in paracancer tissue (0.73±0.14 vs 0.34±0.08, t=17.587, P<0.000) , and the expression level of miR-9 was negatively correlated with HK2; the OD490 level, the expression level of HK2 and the fluorescence activity of double luciferase reporter gene containing HK2 mRNA 3 'UTR in miR-9 group were lower than those in NC group (0.58±0.09 vs 1.04±0.21, 0.51±0.08 vs 1.18±0.24, 41.11±9.28 vs 148.28±29.59, t/P=4.027/0.007, 5.297/0.002, 6.912/0.001) , and the expression level of cleaved caspase-3 was higher than that in NC group (1.08±0.26 vs 0.42±0.09, t/P=4.797/0.003) . The OD490 level and the expression level of HK in HK-siRNA group were higher than that in NC-siRNA group, and the expression level of cleaved caspase-3 was higher than that of NC-siRNA group.
ConclusionmiR-9 can inhibit the proliferation of breast cancer cells, and its mechanism may be related to the targeted inhibition of HK2 expression and the increase of downstream cleaved caspase-3 expression.
Key words:
Breast cancer; miR-9; Hexokinase 2; Target gene; Proliferation
Contributor Information
Wang Yuanbin
Department of Breast Surgery, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
Zhao Yaxin
Yang Lijun
Zheng Jinying
Tang Jundong
Ma Ruiming