Objective To study the changes of zinc and calcium ion in serumand tissue of scalded rats with zinc supplementation. Methods 80 SD rats were randomly divided into control group before scald and N,W,H groups,the rats of N,W,H groups were exposed to dorsal scald injuries of deep skin thickness covering 15% of the total body surface areaa and then were fed on diets containing zinc 40 μg/g in N,W groups and 80 μg/g in H group,a ointment containing zinc 761.1 μg/g was applied on scalded skin in W group at the same time.Zinc and calcium ions concentration in serum,liver,bone,scalded skin were measured on 1、3、7day after scald respectively. Results 1 The serum Zn2+ levels in N,W groups were lower than control before scald (0.66 μg/ml),but it increased obviously in H group (up to 1.06 μg/ml on dl after scald).2. The liver Zn2+ showed increasing tendencies in all groups,It reached 41.7 μg/g on 1 day scald from 22.5 μg/g on before scald in H group and was 1.75 times as much as N group(P<0.01).the contents of calcium in liver increased to 31.7 mg/g from 26.1 mg/g in N,W groups and declined in H group comparing with control.3 The Zn2+ levels in bone showed degression tendencies in all groups from 1 to 7 day after scald,The concentration of bone Zn2+ was from 68.9 μg/g to 41.5 μg/g in N group,which was less 16.7 μg/g than that of H group(P<0.01).Ca2+:level in bone was 73.3mg/g before scald,It was 50.2mg/g in N group on 7 day and less 15.7mg/g comparing with H group(P<0.05)4.the Zn2+ content of scalded skin declined in N,Wgroup,however,the Zn2+ content increased obviously in Hgroup on first day scald,these reached a peak value 34.4μg/g in W group on 7 day,it was 2.8 time as much as before scald.the Ca2+:contents of scald skin display large extent increase in all group,especially in N group,these were 3.0、22.6、66.2 times higher than before scald(0.08mg/g)on 1、3、7 d respectively (P<0.01),But the increase extent in W group was more less level than in N group. Conclusions The contents of Ca2+ were decrease in serum,bone,and increase in live and scalded skin,the cause maybe relate to Ca2+ abnormal distributing in body and calcium overload in cell.Supplement zinc by diet mainly make the Zn2+ levels in serum and liver increase and the Ca2+ contents drop.Supplement zinc by wound can increase Zn2+ content and reduce the Ca2+ level in skin.zinc may posses a role to mitigate calcium overload in cell.