Original Article
Correlations of enterobacteria DNA load in venous blood determined by real-time quantitative polymerase chain reaction with vital signs and blood cell count in febrile surgical patients
Junren Kang, Enling Ma, Wei Chen
Published 2015-10-30
Cite as Chin J Clin Nutr, 2015, 23(5): 292-295. DOI: 10.3760/cma.j.issn.1674-635X.2015.05.007
Abstract
ObjectiveTo determine enterobacteria DNA load in venous blood of febrile surgical patients using real-time quantitative polymerase chain reaction (RQ-PCR), to study the correlations between DNA load and vital signs/blood cell count, and to compare the difference between different detection methods in terms of positive rates.
MethodsA total of 72 blood samples were obtained for bacterial culture and RQ-PCR. The correlations of enterobacteria DNA load with body temperature, heart rate, while blood cell count, and percentages of leukocyte and lymphocyte were then analyzed.
ResultsThe enterobacteria positive rate determined by RQ-PCR (63.89%) was significantly higher than that by bacterial culture (9.72%)(F=4.383, P=0.036). The DNA load was significantly correlated with both body temperature and heart rate (P=0.006, r=0.323; P=0.000, r=0.411), but not with white blood cell count, percentages of leukocyte and lymphocyte, and age(P=0.438, r=0.093; P=0.825, r=0.027; P=0.451, r=-0.090; P=0.096, r=0.198).
ConclusionsRQ-PCR can quickly determine the enterobacteria DNA load in peripheral blood with high sensitivity. Routine blood cell count may not accurately reflect the enterobacteria DNA load in blood. Body temperature and heart rate may be influenced by various factors.
Key words:
Gut barrier injuries; Bacterial translocation; Fever; Enterobacteria; Blood culture; Real-time quantitative polymerase chain reaction
Contributor Information
Junren Kang
Department of Parenteral and Enteral Nutrition, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
Enling Ma
Wei Chen
