Objective To assess the agreement of ACD measurements among Galilei Ⅱ,Pentacam and IOLMaster in myopic eyes.Methods Forty eyes of forty healthy myopia volunteers,whose spherical equivalent were-1.00～-8.00 D,with an average of-4.40±1.80 D,were enrolled in this prospective study.ACD were randomly measured by Galilei Ⅱ,Pentacam and IOLMaster.The differences between ACD measurements by the 3 devices were analyzed by Bonferroni-corrected repeated-measures analysis of variance.Pearson correlation and Bland-Altman analysis were used to assess the correlation and agreement among the 3 measurements.Results The ACD (mean±standard deviation) with Galilei Ⅱ,Pentacam and IOLMaster were 3.81±0.20 mm,3.72±0.20 mm and 3.71± 0.20 mm,respectively.Good correlation were found between any two of 3 devices (r=0.984,0.970,0.968,P＜0.01).The difference between Galilei Ⅱ and Pentacam was 0.10±0.04 mm and statistically significant (P＜0.01).The difference between Galilei Ⅱ and IOLMaster was 0.10±0.05 mm and statistically significant (P＜0.01).The difference between Pentacam and IOLMaster was 0.00±0.05 mm without statistically significant difference (P＞0.05).The 95％ limits of agreement between Galilei Ⅱ and Pentacam,Galilei Ⅱ and IOLMaster,Pentacam and IOLMaster were 0.026～0.167 mm,0.004～ 0.195 mm and-0.096～0.102 mm,respectively.Conclusion In myopic eyes,Pentacam measured similar ACD values with IOLMaster.Galilei Ⅱ measured slightly deeper ACD than either Pentacam or IOLMaster,the differences were within clinically acceptable level.Therefore,they can be regarded clinically interchangeable in measuring myopic eyes.