Original Article
Repeatability of the optical characteristics of the retinal nerve fiber layer measured with optical coherence tomography
Jili Chen, Ce Zheng, Xiaoling Xie, Wenxia Chen, Tingyi Cao, Feiping Xu, Shasha Wang
Published 2015-06-25
Cite as Chin J Optom Ophthalmol Vis Sci, 2015, 17(6): 345-349. DOI: 10.3760/cma.j.issn.1674-845X.2015.06.007
Abstract
ObjectiveTo determine the repeatability of the optical characteristics of the retinal nerve fiber layer (RNFL) measured with optical coherence tomography (OCT) in healthy Chinese individuals.
MethodsIn this cross-sectional study, 50 eyes of 50 normal subjects were consecutively recruited. RNFLs were imaged twice using a Cirrus OCT device with a 5-line raster macular scan and an optic disc cube mode in one day by a single operator. Customized software was used to measure RNFL optical characteristics, including optical density and the attenuation coefficient. Repeatability of OCT and customized software measurements were described by intraclass correlation coefficients (ICCs) , coefficients of variation (COVs) and the Bland-Altman method.
ResultsThe mean and standard deviation of age were 51.2±8.1 years. The mean and standard deviations of macular RNFL thickness, optical density and the attenuation coefficient were 34.38±3.45 μm, 117.90±6.02 grey and 1.99±0.16, respectively. The mean and standard deviations of optic disc RNFL thickness, optical density and the attenuation coefficient were 89.07±3.19 μm, 138.10±18.25 grey and 3.48±0.64, respectively. All the evaluated RNFL parameters had good to excellent reproducibility. ICC ranged from 0.712 to 0.952 and COV ranged from 2.20% to 6.54%. The bias between two OCT measurements were -0.01 grey/0.33 grey and 0.01/-0.01, respectively, for macular and optic disc RNFL optical density and attenuation coefficients. Customized software showed excellent reproducibility in RNFL measurements. ICC ranged from 0.928 to 0.979 and COVs all bellowed 6% (1.35%-4.24%) for RNFL parameters measurement.
ConclusionIn our population of normal adult Chinese eyes, there is good repeatability of RNFL optic characteristics measured by OCT and customized software.
Key words:
Tomography, optical coherence; Repeatability; Retinal nerve fiber layer; Optical characters; Optical density; Attenuate coefficient
Contributor Information
Jili Chen
Shanghai Shibei Hospital, Shanghai 200070, China
Ce Zheng
Xiaoling Xie
Wenxia Chen
Tingyi Cao
Feiping Xu
Shasha Wang