Objective To investigate the teaching effectiveness of pyschological intervention in the teaching of operative surgery. Methods A total of 200 undergraduates were selected among grade 2009 clinical medical majors in Zhuhai Campus of Zunyi Medical College and were randomly divided into observation group and control group, with 100 medical students in each group. The teaching contents, class hour arrangement and teaching link of operative surgery teaching were the same in two groups. The students of observation group were conducted by professional counseling in class and after class. The learning effects of two groups were evaluated and the SCL-90 questionnaire scores of two groups were analyzed and compared. Results of evaluation test and questionnaire were processed and analyzed by SPSS based on Chi square test and Rank sun test. P〈0.05 signifies statistical difference. Results Scores of comprehensive operating examination in observation group were obvi- ously higher than that in control group [（82.21 ± 6.05） vs.（76.29 ± 6.83）]（t=6.49, P=0.000）. SCL-90 scores were significantly lower in observation group than in control group （Z=-3.6254, P=0.000）. Conclusions Application of psychological intervention can improve the teaching quality of operative surgery. Psychological intervention should be integrated into the teaching of operative surgery.