Original Article
Expression of cytokines in the conjunctiva excised from conjunctivochalasis
Minhong Xiang, Hanmin Wang, Yuanling Jia, Huanming Zhou, Xingxing Chen, Xingru Zhang
Published 2017-04-25
Cite as Chin J ocul traum occupat eye dis, 2017, 39(4): 241-244. DOI: 10.3760/cma.j.issn.2095-1477.2017.04.001
Abstract
ObjectiveTo observe the expression of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β) of surgical excised conjunctival tissue from the conjunctivochalasis patients.
MethodsThirty-four patients were divided into 3 groups: conjunctivochalasis group in 15 eyes of 15 cases, age matched patients with pterygium group in 10 eyes of 10 cases and normal control group in 9 eyes of 9 cases. The excised conjunctival tissues of 3 groups were stained with TNF-α and IL-1β immunofluorescence staining. The results were observed and statistically analyzed.
ResultsThe positive cells area of TNF-α in conjunctival tissue from the conjunctivochalasis group was (712.44±33.84) μm2, (650.14±31.76) μm2 from the pterygium group, and (633.25±15.88) μm2 from normal control group, the difference of TNF-α among 3 groups were statistically significant (F=12.729, P=0.000). The positive cells area of IL-1β in conjunctival tissue from the conjunctivochalasis group was (722.11±60.09) μm2, (664.71±33.10) μm2 from the pterygium group, and (627.75±48.83) μm2 from the normal control group, the difference among 3 groups were statistically significant (F=5.648, P=0.013).
ConclusionThe expression of TNF-α and IL-1β in loose conjunctival tissue from conjunctivochalasis is enhanced, which further confirms that inflammation is associated with conjunctivochalasis.
Key words:
Conjunctivochalasis; Conjunctival tissue; Cytokine; TNF-α; IL-1β
Contributor Information
Minhong Xiang
Department of Ophthalmology, Putuo Hospital Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China
Hanmin Wang
Yuanling Jia
Huanming Zhou
Xingxing Chen
Xingru Zhang
