Basic Science Researches
Effect of mechanical stretch on permeability of human pulmonary vascular endothelial cells and underlying molecular mechanism
Wang Ya, Lan Yuan, Fu Wei, Liu Xiaoqing, Huang Yongbo, Mao Pu, Li Yimin
Published 2019-05-28
Cite as Chin J Crit Care Intensive Care Med, 2019,5(2): 151-158. DOI: 10.3877/cma.j.issn.2096-1537.2019.02.013
Abstract
ObjectiveTo observe the effect of mechanical stretch on the permeability of human pulmonary artery endothelial cells (HPAECs) and human pulmonary microvascular endothelial cells (HPMVECs) and to examine whether the expression of the key permeability-related proteins VE-cadherin, Claudin-5, and Caveolin-1 is involved in this process.
MethodsThe mechanical stretch device Flexcell FX-5000T was used to apply 10% or 20% tensile stress to HPAECs and HPMVECs at a frequency of 0.5 Hz. qRT-PCR and Western blot were used to detect the mRNA and protein expression of VE-cadherin, Claudin-5, and Caveolin-1 in cells before and after mechanical stretch. Mechanical stretched HPAEC cells and HPMVEC cells were monitored by a cell dynamic analyzer and Transwell chamber/FITC-albumin assay to detect their permeability.
ResultsIn HPAECs after mechanical stretch, the expression of VE-cadherin mRNA and protein, Claudin-5 mRNA and protein, and Caveolin-1 mRNA decreased significantly compared with control cells (P<0.05), while the level of Caveolin-1 protein did not change significantly compared with the control group. In HPMVECs after mechanical stretch, the expression of VE-cadherin mRNA and protein and Caveolin-1 mRNA and protein decreased significantly compared with control cells (P<0.05), while the levels of Claudin-5 mRNA and protein did not change significantly compared with the control group.
ConclusionMechanical stretch leads to increased permeability of pulmonary vascular endothelial cells, which is caused by down-regulation of the expression of permeability key proteins, but the permeability mechanisms of the two cell types are different: HPAECs involve tight junctions and HPMVECs involve the transendothelial pathway.
Key words:
Mechanical stretch; Human pulmonary artery endothelial cells; Human pulmonary microvascular endothelial cells; VE-cadherin; Claudin-5; Caveolin-1
Contributor Information
Wang Ya
The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Diseases, Guangzhou 510120, China
Lan Yuan
The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Diseases, Guangzhou 510120, China
Fu Wei
The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Diseases, Guangzhou 510120, China
Liu Xiaoqing
The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Diseases, Guangzhou 510120, China
Huang Yongbo
The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Diseases, Guangzhou 510120, China
Mao Pu
Department of Infection Management, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China
Li Yimin
The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Diseases, Guangzhou 510120, China
