Basic Science Research
Preparation of IR780 and vincristine-loaded poly (lactic-co-glycolic acid) nanoparticles and antinep-hroblastoma cells proliferation in vitro
Xu Xiaochuan, Tang Yi, Chen Jingyu, Ji Xiaojuan, Yang Chunjiang, Zhu Lirong
Published 2020-04-01
Cite as Chin J Med Ultrasound(Electronic Edition), 2020,17(04): 363-369. DOI: 10.3877/cma.j.issn.1672-6448.2020.04.013
Abstract
ObjectiveTo prepare IR780 and vincristine (VCR)-loaded poly(lactic-co-glycolic acid)(PLGA) nanoparticles (IR780-VPN) and verify their specific targeting ability and anti-proliferative efficiency in vitro.
MethodsIR780-VPN were prepared to detect their basic physical and chemical characteristics. SK-NEP1 cells were used as a tumor cell model to verify the specific targeting ability and the anti-proliferative efficiency of IR780-VPN. SK-NEP1 cells were divided into six groups: group A: PBS; group B: free VCR; group C: PLGA nanoparticles; group D: VCR-PLGA nanoparticles; group E: IR780-PLGA nanoparticles; group F: IR780-VPN.
ResultsThe average particle size of IR780-VPN was (290.82±3.22) nm, the particle dispersion index (PDI) was 0.024, and the average zeta potential of IR780-VPN was (1.16±0.87) mV. IR780-VPN showed stable physical and chemical properties, and they were spherical, uniform in size, and regular in shape, without aggregation oradhesion under a microscope. The morphology of IR780-VPN was spherical or quasi-spherical with a smooth surface, and the particle size distribution was uniform. The average encapsulation efficiency of IR780-VCR-PLGA nanoparticles was about 72.80%, the average loading efficiency was 2.80%, and the specific targeting efficiency was 91.30%. Many IR780-VPN were observed to target tumor cells in vitro. The cytotoxicity was the highest in group F when compared with group B and group D at the same concentration (P<0.05), and cytotoxicity was concentration-dependent in group B, group D, and group F: the higher the drug concentration, the stronger the cytotoxicity (F=13254.105, 54354.510, and 1370.059, P<0.05). There was no significant difference in group C and group E with the increase of drug concentration (P>0.05). The rate of reduced cell proliferation in group A was (2.83±0.32)%.
ConclusionIR780-VPN with stable properties have been successfully prepared, which can specifically target tumor cells and have improved anti-proliferative efficiency in vitro.
Key words:
Targeted nanoparticles; Ultrasound contrast agents; Wilms tumor; Cell proliferation
Contributor Information
Xu Xiaochuan
Department of Ultrasound, Ministry of Education Key Laboratory of Child Development and Disorders; National Clinical Research Center for Child Health and Disorders (Chongqing); China International Science and Technology Cooperation Base of Child Development and Critical Disorders; Chongqing Key Laboratory of Pediatrics; Children′s Hospital of Chongqing Medical University, Chongqing 400014, China
Tang Yi
Department of Ultrasound, Ministry of Education Key Laboratory of Child Development and Disorders; National Clinical Research Center for Child Health and Disorders (Chongqing); China International Science and Technology Cooperation Base of Child Development and Critical Disorders; Chongqing Key Laboratory of Pediatrics; Children′s Hospital of Chongqing Medical University, Chongqing 400014, China
Chen Jingyu
Department of Ultrasound, Ministry of Education Key Laboratory of Child Development and Disorders; National Clinical Research Center for Child Health and Disorders (Chongqing); China International Science and Technology Cooperation Base of Child Development and Critical Disorders; Chongqing Key Laboratory of Pediatrics; Children′s Hospital of Chongqing Medical University, Chongqing 400014, China
Ji Xiaojuan
Department of Ultrasound, Ministry of Education Key Laboratory of Child Development and Disorders; National Clinical Research Center for Child Health and Disorders (Chongqing); China International Science and Technology Cooperation Base of Child Development and Critical Disorders; Chongqing Key Laboratory of Pediatrics; Children′s Hospital of Chongqing Medical University, Chongqing 400014, China
Yang Chunjiang
Department of Ultrasound, Ministry of Education Key Laboratory of Child Development and Disorders; National Clinical Research Center for Child Health and Disorders (Chongqing); China International Science and Technology Cooperation Base of Child Development and Critical Disorders; Chongqing Key Laboratory of Pediatrics; Children′s Hospital of Chongqing Medical University, Chongqing 400014, China
Zhu Lirong
Department of Ultrasound, Ministry of Education Key Laboratory of Child Development and Disorders; National Clinical Research Center for Child Health and Disorders (Chongqing); China International Science and Technology Cooperation Base of Child Development and Critical Disorders; Chongqing Key Laboratory of Pediatrics; Children′s Hospital of Chongqing Medical University, Chongqing 400014, China
