Original Article
Effect of electroacupuncture at Zusanli point on gastric emptying rate and gastric mucosal blood flow after oral resuscitation of full thickness scalded rats
Zhang Huiping, Zhao Zengkai, Lyu Yi, Ma Liqian, Wang Haibin, Zhang Lijian, Zhou Fangqiang, Cai Hongjuan, Hu Sen
Published 2020-02-01
Cite as Chin J Injury Repair and Wound Healing(Electronic Edition), 2020,15(01): 45-50. DOI: 10.3877/cma.j.issn.1673-9450.2020.01.007
Abstract
ObjectiveTo study the effect of electroacupuncture at Zusanli point on gastric emptying rate and mucosal blood flow after oral rehydration of full thickness scalded rats.
MethodsUsing random number table method, 120 rats were divided into 5 groups: sham+ intragastric rehydration group(GR group), scald+ intragastric rehydration group(SGR group), scald+ electro-acupuncture+ intragastric rehydration group(SGR+ EA group), vagotomy+ scald+ intragastric rehydration group(VGX+ SGR group) and vagotomy+ scald+ electro-acupuncture+ intragastric rehydration group (VGX+ SGR+ EA group), with 24 rats in each group. In 1 000 mL of sterile distilled water, 3.5 g of sodium pyruvate, 3.5 g of sodium chloride, 1.5 g of potassium chloride, and 20.0 g of glucose were added, sodium pyruvate oral rehydration salt (Pyr-ORS) was configured, and the pH was adjusted to 7.35. After the rats were anesthetized, the carotid artery, femoral vein, and femoral artery were separated and the catheterization was did. The scalded rats were scalded with boiling water (100 ℃, 15 s) for about 35% total body surface area (TBSA) of the back, and the scalded rats were immersed in warm water (37 ℃, 15 s). Immediately after the scald, each rat was given a subcutaneous injection of 0.5 mL of 0.9% sodium chloride solution and 500 mL of buprenorphine for pain relief. Pyr-ORS was immediately infused via a gastric tube using an infusion pump for 1 h. Rats in the VGX+ SGR group and the VGX+ SGR+ EA group underwent bilateral vagus nerve resection before burns. In the SGR+ EA and VGX+ SGR+ EA groups, Zusanli point was selected for acupuncture and connected to an electroacupuncture instrument. The intensity was 2 mA, 2 to 100 Hz, and the acupuncture depth was 7 mm. After rehydration, the electroacupuncture was continued for 0.5 h. The gastric emptying rate and gastric mucosal blood flow of each group were measured at 2 and 4 h after scald. Data were compared using one-way analysis of variance and t test.
ResultsThe gastric emptying rates of rats in the SGR group, SGR + EA group, VGX + EA group, and VGX + SGR + EA group [2 h: (16.5±3.2)%, (22.6±3.7)%, (13.6±3.0)%, (14.2±3.2)%; 4 h: (12.9±2.4)%, (17.1±2.3)%, (10.8±2.7)%, (12.7±2.5)% were lower than those of rats in the GR group [2 h: (45.9±4.6)%; 4 h: (42.4±5.7)%], the differences were statistically significant [2 h: (t=18.18, 13.67, 20.37, 19.60; with P values below 0.05); 4 h: (t=28.29, 24.71, 28.67, 27.96; with P values below 0.05). The gastric emptying rates of rats in the SGR+ EA group was higher than those of rats in the SGR group, VGX+ SGR group and VGX+ SGR+ EA group, and the differences were statistically significant [2 h: (t=4.32, 6.54, 5.94; with P values below 0.05); 4 h: (t=4.38, 6.15, 4.49; with P values below 0.05)]. Gastric mucosal blood flow of rats in the SGR group, SGR+ EA group, VGX+ EA group, and VGX+ SGR+ EA group at 2 and 4 h after scald [2 h: (54.0±7.3), (74.6±8.7), (42.0±5.5), (45.2±6.2) BPU, 4 h: (46.0±6.8), (65.6±10.1), (37.8±5.6), (42.7±6.4) BPU] were significantly lower than the GR group [2 h: (142.0±18.1) BPU; 4 h: (127.4±16.5) BPU], the differences were statistically significant [2 h: (t=15.67, 11.67, 18.35, 17.56; with P values below 0.05); 4 h: (t=15.80, 11.07, 17.81, 16.58; with P values below 0.05)]; the gastric mucosal blood flow of rats in the SGR+ EA group compared with SGR group, VGX+ SGR group and VGX+ SGR+ EA group, the difference were statistically significant[2 h: (t=6.28, 10.97, 9.53; with P values below0.05); 4 h: (t= 5.58, 8.33, 6.63; with P values below 0.05)].
ConclusionsElectro acupuncture at Zusanli point can promote the rate of gastric emptying, increase gastric mucosal blood flow, and improve the efficacy of oral rehydration in full thickness scalded rats. The mechanism may be related to the stimulation of the vagus nerve.
Key words:
Electroacupuncture; Rats; Fluid therapy; Vagus nerve; Scald; Zusanli; Gastric emptying
Contributor Information
Zhang Huiping
Research Center of Trauma Repair and Tissue Regeneration, Medical Innovation Research Department, Chinese PLA General Hospital, Beijing 100853, China
Zhao Zengkai
Department of Acupuncture, Dong Zhi Men Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Lyu Yi
Research Center of Trauma Repair and Tissue Regeneration, Medical Innovation Research Department, Chinese PLA General Hospital, Beijing 100853, China
Ma Liqian
Research Center of Trauma Repair and Tissue Regeneration, Medical Innovation Research Department, Chinese PLA General Hospital, Beijing 100853, China
Wang Haibin
Department of Laboratory Medicine, Fourth Medical Center of Chinese PLA General Hospital, Beijing 100037, China
Zhang Lijian
Department of Realbilitation, Eighth Medical Center of Chinese PLA General Hospital, Beijing 100094, China
Zhou Fangqiang
Three Generations Pharmaceutical Technology Co., Ltd, Shanghai 200020, China
Cai Hongjuan
Medical Intensive Care Unit, Peking Union Medical College Hospital, Beijing 100730, China
Hu Sen
Research Center of Trauma Repair and Tissue Regeneration, Medical Innovation Research Department, Chinese PLA General Hospital, Beijing 100853, China