Original Article
Value of preoperative levels of serum miR-20a and miR-17 in the prognosis assessment of patients with colorectal cancer
Chen Jiyun, Zhou Shilin, Xiong Danli, Wang Lingli, Ma Peifang, Zhang Jingjing, Zhang Wanli, Xiao Yong, Niu Yanfeng
Published 2020-04-01
Cite as Chin Arch Gen Surg(Electronic Edition), 2020,14(02): 107-110. DOI: 10.3877/cma.j.issn.1674-0793.2020.02.007
Abstract
ObjectiveTo investigate the value of preoperative levels of serum miR-20a and miR-17in the evaluation of adverse prognosis of patients with colorectal cancer.
MethodsFrom August 2013 to February 2016, seventy-three patients with colorectal cancer in Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology were selected as case group, and the serum samples were collected before operation, and 1 week, 1 month, 3 months after operation. Serum specimens of 81 healthy persons as control group were collected during the same period. According to the average level of miR-20a and miR-17 expression detected by fluorescent quantitation PCR, they were divided into high expression group and low expression group and survival analyzed by Kaplan-Meier method. Cox proportional hazard regression model was used to screen the influencing factors of poor prognosis. The diagnostic efficacy of miR-20a and miR-17 in the poor prognosis of colorectal cancer was analyzed by ROC curve.
ResultsThe serum levels of miR-20a and miR-17 in the case group decreased after operation (P<0.05), and the levels of each detection point were higher than those in the control group (P<0.05). Differentiation (HR=1.462, 95% CI: 1.096-1.951), TNM stage (HR=1.642, 95% CI: 1.339-2.014), preoperative serummiR-20a level (HR=1.575, 95% CI: 2.035-3.652) and miR-17 level (HR=2.491, 95% CI: 2.131-2.914) were independent risk factors affecting the poor prognosis of colorectal cancer patients (all P<0.001). The 3-year overall survival rate of preoperative patients with high expression of serum miR-20a and miR-17 was lower (22.0% vs 78.3%, 21.3% vs 73.1%, P<0.05), and the area under the curve for predicting poor prognosis of patients with colorectal cancer was 0.955, the sensitivity was 97.6%, and the specificity was 91.2%.
ConclusionsThe high expression of serum miR-20a and miR-17 in patients with colorectal cancer before operation is related to poor prognosis. Preoperative detection is helpful to improving the clinical outcomes of patients with high risk of poor prognosis.
Key words:
Colorectal neoplasms; MicroRNAs; Prognosis; miR-20a; miR-17
Contributor Information
Chen Jiyun
Department of Gastrointestinal Surgery, Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China
Zhou Shilin
Department of General Surgery, Jiajiang Hospital of Traditional Chinese Medicine, Leshan 614100, China
Xiong Danli
Department of Gastrointestinal Surgery, Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China
Wang Lingli
Department of Gastrointestinal Surgery, Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China
Ma Peifang
Department of Gastrointestinal Surgery, Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China
Zhang Jingjing
Department of Gastrointestinal Surgery, Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China
Zhang Wanli
Department of Gastrointestinal Surgery, Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China
Xiao Yong
Department of Gastrointestinal Surgery, Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China
Niu Yanfeng
Department of Gastrointestinal Surgery, Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China
