Basic Science Research
Expression and role of platelet-derived growth factor D in tongue squamous cell carcinoma
Quan Wan, Jiuyang Jiao, Zhiyuan Lu, Chaobin Pan
Published 2019-06-01
Cite as Chin J Stomatol Res(Electronic Edition), 2019, 13(3): 151-157. DOI: 10.3877/cma.j.issn.1674-1366.2019.03.004
Abstract
ObjectiveTo explore the role of platelet-derived growth factor D (PDGF-D) in human tongue squamous cell carcinoma (TSCC) .
MethodsIn total, 127 human tongue carcinoma specimens and 15 adjacent tumor specimens were tested for PDGF-D with immunohistochemical approaches. T-test was used to analyze the relationship between the expression of PDGF-D and the age, gender, TNM stage, clinical stage of patients. Survival analysis was conducted in a proportional hazards model. PDGF-D overexpressed and knockdown cells were constructed. The proliferative, migrative, and invasive ability of TSCC cells was assessed in vitro. Epithelial-mesenchymal transition (EMT) related proteins were tested.
ResultsImmunohistochemical results demonstrated that the expression of PDGF-D was higher in cancer tissue than that of normal tissue (P<0.001) . Chi-square test showed that the expression of PDGF-D was related to the TNM stage, clinical stage and lymph node metastasis of TSCC patients. Survival analysis showed that patients with low PDGF-D expression were expected to have longer survival time (P=0.0446) . PDGF-D may act as an independent risk factor (HR=2.643, P<0.05) . PDGF-D overexpressed cells formed more colonies in the colony forming test (P<0.001) , and showed more penetrated cells in the transwell migration (P=0.044) and invasion (P<0.001) tests. By contrast, PDGF-D knocked down cells formed less colonies in the colony forming test (P=0.026) , and showed less penetrated cells in the transwell migration (P<0.001) and invasion (P<0.001) tests. Western blot showed that the epithelial protein marker (E-cad) was depressed while the expression of mesenchymal markers (N-cad, Vimentin) was upregulated in PDGF-D overexpressed cells. The effect of knocking down PDGF-D on TSCC cell and EMT was in the reverse way.
ConclusionsThe expression of PDGF-D was strongly expressed in tongue carcinoma and correlated with its prognosis. PDGF-D enhanced the proliferation, migration, and invasion of TSCC cells. Statistically, PDGF-D may act as an independent prognostic factor for TSCC.
Key words:
Platelet-derived growth factor; Carcinoma, squamous cell; Tongue; Neoplasm metastasis; Epithelial-mesenchymal transition
Contributor Information
Quan Wan
Department of Oral and Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
Jiuyang Jiao
Department of Oral and Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
Zhiyuan Lu
Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
Chaobin Pan
Department of Oral and Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
