Original Article
Experimental study of lncRNA PACER promoting inflammatory response of acute lung injury induced by sepsis
Liuyan Shi, Wei Xin, Quanfang Wei, Zhixin Gan, Dan Wang, Mingdong Hu
Published 2019-02-20
Cite as Chin J Lung Dis(Electronic Edition), 2019, 12(1): 38-42. DOI: 10.3877/cma.j.issn.1674-6902.2019.01.007
Abstract
ObjectiveTo investigate the effect of lncRNA PACER on inflammatory response of acute lung injury induced by sepsis in mice.
MethodsHuman alveolar macrophages were collected from 20 cases of acute lung injury and health nonsmokers, and Lung tissues of ALI mice induced by LPS were obtained to detect the expression of lncRNA PACER by qRT-PCR. Then, lncRNA PACER was overexpressed or knocked down in THP-1 and RAW264.7 cells, the expressions of cytoinflammatory cytokines TNF-α and IL-6 were detected by ELISA. Furthermore, the ALI mice induced by LPS were injected PACER siRNA lentivirus into the tail vein, and the expressions of serum inflammatory factors TNF-α and IL-6 were detected by ELISA, meanwhile, the pathologic injury of lung tissue were observed by HE staining.
ResultsThe expression of lncRNA PACER was significantly increased in both alveolar macrophages of ALI patients and in lung tissues of ALI mice (P<0.01). After overexpression of PACER, the expression of inflammatory cytokines TNF-α and IL-6 were increased, while the expression of inflammatory cytokines TNF-α and IL-6 decreased after knocked down PACER (P<0.01). The expression of inflammatory cytokines TNF-α and IL-6 in lung tissue and serum of ALI mice were significantly decreased after the mice were knocked down PACER (P<0.01), and the pathological damage in lung tissues was significantly alleviated in PACER knockdown mice.
ConclusionsLncRNA PACER significantly promotes the inflammatory response of acute lung injury in sepsis, which provides basis for the identification of lncRNA PACER as a target for the prevention and treatment of ALI.
Key words:
LncRNA PACER; Sepsis; Acute lung injury; Inflammatory cytokines
Contributor Information
Liuyan Shi
Department of Scientific Research and Education, The Affiliated Baoji Hospital, Xi′an Medical University, Baoji 721006, China
Wei Xin
Department of Thoracic Surgery, Baoji Traditional Chinese Medicine Hospital, Baoji 721001, China
Quanfang Wei
Biomedical Analysis Center, Army Medical University, Chongqing 400038, China
Zhixin Gan
Department of Respiratory and Critical Care Medicine, XinQiao Hospital, Army Medical University, Chongqing 400037, China
Dan Wang
Department of Respiratory and Critical Care Medicine, XinQiao Hospital, Army Medical University, Chongqing 400037, China
Mingdong Hu
Department of Respiratory and Critical Care Medicine, XinQiao Hospital, Army Medical University, Chongqing 400037, China
