Basic Researches
Contact regulation of bone marrow mesenchymal stem cells on hepatic stellate cells
Musitaba· Maimaitireyimu, Chen Jingyao, Lin Nan, Cao Yanlong, Wu Jianhua, Lin Yang
Published 2020-02-10
Cite as Chin J Hepat Surg(Electronic Edition), 2020,09(01): 83-86. DOI: 10.3877/cma.j.issn.2095-3232.2020.01.018
Abstract
ObjectiveTo investigate the direct contact regulation of bone marrow mesenchymal stem cells (BMSCs) upon hepatic stellate cells (HSCs).
MethodsThe co-culture system of HSC and BMSC (co-culture group) was established. After 72-h co-culture, the cells were isolated by flow cytometry. The expression levels of related receptors of Notch signaling pathway (Notch1, Notch2, Notch3 and Notch4) in HSCs and Notch-related ligands (DII1, DII3, DII4, Jag1 and Jag2) in BMSCs from the co-culture group were detected by Western blot. HSCs and BMSCs which were cultured separately were taken as the control group. The expression levels of proteins were statistically compared between two groups using t test.
ResultsIn the co-culture group, the expression level of Notch1 was 1.85±0.34, significantly higher than 0.98±0.25 in the control group (t=4.235, P<0.05). However, the expression of Notch3 was 0.40±0.12, significantly lower than 1.00±0.08 in the control group (t=-7.971, P<0.05). The expression level of Notch-related ligand Jag1 was 2.03±0.30, significantly higher compared with 0.99±0.13 in the control group (t=6.386, P<0.05).
ConclusionBMSCs may regulate HSCs through combining Jag1 with Noch1 in a direct contact way.
Key words:
Mesenchymal stem cells; Bone marrow; Hepatic stellate cell; Coculture techniques
Contributor Information
Musitaba· Maimaitireyimu
Department of General Surgery, the First People's Hospital of Kashgar Prefecture, Kashgar Prefecture 844000, China
Chen Jingyao
Digestive Medicine Center, the Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen 518107, China
Lin Nan
Department of General Surgery, the First People's Hospital of Kashgar Prefecture, Kashgar Prefecture 844000, China; Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
Cao Yanlong
Department of General Surgery, the First People's Hospital of Kashgar Prefecture, Kashgar Prefecture 844000, China
Wu Jianhua
Department of General Surgery, the First People's Hospital of Kashgar Prefecture, Kashgar Prefecture 844000, China
Lin Yang
Department of General Surgery, the First People's Hospital of Kashgar Prefecture, Kashgar Prefecture 844000, China
