Experts Forum
Application and quality management of high-throughput sequencing in clinical molecular diagnosis of five kinds of diseases
Yun Bao, Yanqun Xiao, Hualiang Wang
Published 2018-05-28
Cite as Chin J Clin Lab Mgt (Electronic Edition), 2018, 06(2): 69-73. DOI: 10.3877/cma.j.issn.2095-5820.2018.02.002
Abstract
High-throughput sequencing, also known as the high-throughput next generation sequencing (NGS), can sequence thousands of genes at one time, greatly improve the efficiency of DNA sequencing and reduce the cost. Recently, NGS has been widely used in medical research and clinical diagnosis. It has extensive applications in non-invasive prenatal screening, tumor screening, diagnosis and treatment, microbial pathogens detection, genetic diseases screening and diagnosis as well as organ transplantation rejection screening. However, as a new technology, NGS also has many issues in related clinical application. The quality management system is still imperfect and faces many challenges. Thus the joint efforts of medical laboratories and related departments are needed to promote the standardization of NGS in clinical diagnosis.
Key words:
Next generation sequencing technology; Molecular diagnosis; Quality management
Contributor Information
Yun Bao
Department of Molecular Biology, Shanghai Center for Clinical Laboratory, Shanghai 200126, China
Yanqun Xiao
Hualiang Wang