Clinical Research
Determination of plasma warfarin concentration by ultra-high performance liquid chromatography and clinical application
Jiangtao Tang, Yao Chen, Yangjuan Bai, Qiang Miao, Yuangao Zou
Published 2019-08-28
Cite as Chin J Clin Lab Mgt (Electronic Edition), 2019, 07(3): 133-136. DOI: 10.3877/cma.j.issn.2095-5820.2019.03.002
Abstract
ObjectiveTo establish a method to determine warfarin concentration in plasma by ultra performance liquid chromatography (UPLC), and to explore the relationship between warfarin plasma concentration and international normalized ratio (INR) in patients after cardiac valve replacement, so as to seek more safe and reliable monitoring indicators and guide rational use of anticoagulant therapy.
MethodsAn ultra performance liquid chromatography method has been developed for measuring warfarin concentration in plasma by using 6-methoxy naphthaleneacetic acid (6-MNA) as internal standard (IS). At the some time, the correlation of INR, warfarin dosage and plasma warfarin concentration in 79 samples was analyzed.
ResultsThe retention time of warfarin and the IS were 2.2 and 1.1 min, respectively. The average extraction recovery of plasma warfarin was 96.0%, The concentration of warfarin was 15.6~4000 ng/ml with good linearity (R2=0.9996). The overall accuracy of the method was 98.5%~99.9% and the lowest detection limit was 5.0 ng/ml. The intra-and inter-day variations were less than 1.34% and 2.69%, respectively. The correlation between INR and warfarin dose was r2=0.006 (P=0.481), INR and plasma warfarin concentration was r2=0.006 (P=0.497), and the correlation between warfarin dose and plasma concentration was r2=0.298 (P=0.001) in 79 cases of samples.
ConclusionsA simple, rapid, accurate and sensitive UPLC method for quantifying warfarin levels in human plasma was validated. The determination of warfarin blood concentration has guiding significance for anticoagulant therapy of patients after heart valve replacement.
Key words:
Warfarin; International normalized ratio; Ultra performance liquid chromatography
Contributor Information
Jiangtao Tang
Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu 610041, China
Yao Chen
Department of Clinical Laboratory, Women′s and Children′s Hospital of Sichuan Province, Chengdu 610000, China
Yangjuan Bai
Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu 610041, China
Qiang Miao
Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu 610041, China
Yuangao Zou
Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu 610041, China