The essential defect of von Willebrand disease is the qualitative or/and quantitative abnormality of the vWF (von Willebrand factor) . This paper reported the successful PCR amplification of 5overlaping fragments of vWF cDNA from the total RNA of the human umbilical vein endothelial cells and the assembling of them into pGEM-3Zf (+) and pBluescriptSK (+) . The finally assembled product was 8515bp in length and contained the whole coding region of vWF cDNA. The result of enzyme-cutting analysis of this vWF cDNA conformed to what was expected according to published sequence data. The authenticity of this gene's reading-frame was determined in TNT translation system and vWF antigen was revealed both in CHO cells and in the supernatant after transfection with the vWF cDNA inserted pCI eukaryotic expression vector.

von Willebrand factor; Polymerase chain reaction; DNA cloning; In vitro translation; Eukaryotic expression