Applied Study and Case Report
Effects on the respiration and nervous system of rat exposed to the thermobaric bomb simulated gas
Wang hong, Gao Junhong, Liu Zhiyong, Lu Qing, Gao Yongchao, Niu Hao, Yue hong
Published 2019-11-20
Cite as Chin Ind Hyg Occup Dis, 2019,37(11): 815-819. DOI: 10.3760/cma.j.issn.1001-9391.2019.11.004
Abstract
ObjectiveTo investigate the effects of simulated-thermobaric explosive gas on the respiration and nervous system in rats.
Methods70 of SPF SD rats were randomly divided into four thermobaric explosive gas groups, two restoration observation groups and control group from April to August in 2018. The exposure time of in four thermobaric explosive gas groups were 3.75, 7.5, 15.0 and 30 min, respectively. The restoration observation groups were designed to observe for 30 and 120 min after exposure thermobaric explosive gas 30 min. The bloods were collected and analyzed at the end of exposure and recovery observation. The endogenous carbon monoxide (CO) , nitric oxide (NO) , glutamic acid (GLU) , acetylcholinesterase (AchE) and dopamine (DA) were detected in brain tissues, respectively.
ResultsThe blood gas index (pH, PCO2, PO2, COHb, O2Hb, MeHbt) and blood electrolytes (Na+, K+, Ca2+ and Cl-) in exposure groups have significant differences with these in control (P<0.05) . The pH value decreased with the exposure time longer. However, it basically returned to normal level when terminating exposure for 120 min. The concentration of PCO2, MeHb and CoHb increased first and then decreased with the exposure time extension. Conversely, The PO2 and O2Hb decreased first and then increased with the exposure time longer. The concentration of endogenous CO, GLU, and AchE decreased and NO increased in exposure group 4 and the restoration observation group 1 compared with those in control (P<0.01) . In addition, there were pathological changes in lung and brain tissue of exposure group, such as inflammatory cell infiltration and edema.
ConclusionThe blood gas index, electrolytes, neurotransmitter, histopathology of lung and brain were changed to various degrees by thermobaric bomb gas exposure. These findings would provide some beneficial support for evaluating the damage effect of thermobaric bomb gas on organisms.
Key words:
Thermobaric Bomb; Blood; Electrolyte; Neurotransmitter; Rat
Contributor Information
Wang hong
Laboratory of Toxicology and Biological Effects, Institute for Hygiene of ordnance Industry, Xi'an 710065, China
Gao Junhong
Liu Zhiyong
Lu Qing
Gao Yongchao
Niu Hao
Yue hong