Special Issue of Tumor Monograph
Expression and function of miR-346 in colon cancer cells
Liu Haitao, Ma Yingjie, Gao Xiaozhuo, Yan Xiaofei, Zhang Rui, Ma Siping, Liu Fang, Shi Gang
Published 2019-01-01
Cite as Clin Med Chin, 2019,35(1): 18-21. DOI: 10.3760/cma.j.issn.1008-6315.2019.01.005
Abstract
ObjectiveTo detect the expression of miR-346 in colon cancer cells and the relationship with invasion and metastasis, so as to provide experimental basis for predicting and treating targets of colon cancer.
MethodsQuantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of microRNA-346 in colon cancer cell lines (HCT116, COLO205, SW620) and normal colon epithelial cells (NCM460). The expression of microRNA-346 was up-regulated or down-regulated by mimic or inhibitor and transfected into HCT116 cells.The transfection efficiency was detected by qRT-PCR.The metastasis and infiltration behavior of HCT116 cells were observed by Transwell.
ResultsThe relative expression of microRNA-346 in colon cancer cell lines HCT116, COLO205 and SW620 were (0.372±0.068), (1.284±0.253), (1.105±0.203), respectively.The relative expression of COLO-205 and SW620 in normal colon epithelial cells (NCM460) was (0.126±0.004). Compared with NCM460 group, COLO-205 group and SW620 group had significant difference (t=9.652, P<0.01; t=7.753, P<0.01). The invasion and metastasis ability of HCT116 cells increased after the up-regulation of the expression of microRNA-346 (t=2.458, P<0.05; t=2.194, P<0.05), and decreased after the down-regulation of the expression of microRNA-346 (t=2.247, P<0.05; t=2.065, P<0.05).
ConclusionMir-346 is highly expressed in colon cancer cells and regulates invasion and metastasis of colon cancer cells.
Key words:
MiR346; Colon cancer; Metastasis; Invasion
Contributor Information
Liu Haitao
General Surgery, Huludao Central Hospital, Huludao 125001, China
Ma Yingjie
General Surgery, Huludao Central Hospital, Huludao 125001, China
Gao Xiaozhuo
Pathological Center, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang 110042, China
Yan Xiaofei
Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang 110042, China
Zhang Rui
Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang 110042, China
Ma Siping
Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang 110042, China
Liu Fang
Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang 110042, China
Shi Gang
Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang 110042, China
