Special Issue of Tumor Monograph
Predictive value of in vitro methyl thiazolyl tetrazolium assay chemosensitivity test of cytotoxic drug activity in breast cancer
Xu Meiyu, Shen Ye, Jiang Yeping, Shan Yuanzhou, Wu Tao
Published 2019-01-01
Cite as Clin Med Chin, 2019,35(1): 5-10. DOI: 10.3760/cma.j.issn.1008-6315.2019.01.002
Abstract
ObjectiveTo investigate the value of methyl thiazolyl tetrazolium assay (MTT) in predicting drug sensitivity of breast cancer cells in vitro.
MethodsFrom January 2010 to July 2016, one hundred and ninety-two patients with breast cancer who underwent modified radical mastectomy or breast conserving surgery (no preoperative radiotherapy or chemotherapy) in the Shanghai Fengxian District Central Hospital were selected.MTT method was used to determine the inhibitory level and sensitivity of 12 drugs and 3 chemotherapy regimens to primary cultured cancer cells of 192 patients with breast cancer.
Results(1)The sensitivity of breast cancer cells to 12 drugs were in sequence from high to low as follows: Paclitaxel (PTX) > Epirubicin (EPI) > Cisplatin (DDP) > 5-Fluorouracil (5-FU) > Mitoxantrone (MIT) >Vincristine (VCR) > Pirarubicin (THP) > Isosophosphamide (IFO) > Carboplatin (CBP) >Cyclophosphamide (CTX) > Methotrexate (MTX) > Changchun Rui bin (NVB). The sensitivity of chemotherapy regimens in the three groups from high to low was docetaxel/doxorubicin/cyclophosphamide (TAC)>cyclophosphamide/epirubicin/fluorouracil (CEF)>cyclophosphamide/methotrexate/fluorouracil (CMF). The sensitivity rates of PTX, EPI and DDP were 54% (104/192), 42% (81/192) and 37% (71/192) respectively.(2) The average inhibitory rates of DDP, CBP and MIT in stage III breast cancer was higher than those in stage I and II breast cancer, and the differences were statistically significant (F=11.14, 4.303, 3.182, P<0.05). (3) HR-breast cancer is more sensitive than HR+ breast cancer, PTX, EPI, THP, MIT in HER-2(+ ) breast cancer is more sensitive than in HER-2(-)breast cancer.
ConclusionAs a widely used drug sensitivity test method, MTT assay has a certain reference value for screening sensitive drugs and selecting clinical chemotherapy regimens in neoadjuvant chemotherapy of breast cancer.PTX, EPI and DDP are more sensitive to other breast cancer cells than other drugs.Chemotherapy based on in vitro susceptibility results improves the efficiency of chemotherapy and decreases the proportion of changes in chemotherapy schemes due to inefficiency.
Key words:
MTT assay; Breast cancer; Cell; Chemosensitivity test
Contributor Information
Xu Meiyu
Department of General Surgery, Shanghai Fengxian District Central Hospital, Shanghai 201499, China
Shen Ye
Jiang Yeping
Shan Yuanzhou
Wu Tao
