Pain Management and Research
Role of Sonic hedgehog signaling pathway in spinal cord in neuropathic pain in rats
Xiaoxue Feng, Danyan Liu, Xiaoqiu Yang
Published 2016-02-20
Cite as Chin J Anesthesiol, 2016, 36(2): 175-179. DOI: 10.3760/cma.j.issn.0254-1416.2016.02.014
Abstract
ObjectiveTo evaluate the role of Sonic hedgehog (Shh) signaling pathway in the spinal cord in neuropathic pain (NP) in the rats.
MethodsSeventy-two male Sprague-Dawley rats, aged 8 weeks, weighing 250-300 g, were randomly divided into 2 groups (n=36 each) using a random number table: sham operation group (S group) and NP group.Spared nerve injury was produced by exposing the sciatic nerve and its branches and ligation and transection of tibial nerve and common fibular nerve in anesthetized rats.The mechanical paw withdrawal threshold (MWT) was measured before operation and at 1, 4, 7, 14 and 21 days after operation.After measurement of the pain threshold at 1, 4, 7, 14 and 21 days after operation, the animals were then sacrificed, and the lumbar segment (L4-6) of the spinal cord was obtained for determination of Shh, Patched(Ptch), Gli1 and glial fibrillary acidic protein (GFAP) expression (by Western blot), Shh, Ptch and Gli1 mRNA expression (by fluorescent quantitative real-time reverse transcriptase-polymerase chain reaction), and Shh and GFAP expression (by immunohistochemistry).
ResultsCompared with group S, the MWT was significantly decreased, and the expression of Shh, Ptch and Gli1 protein and mRNA and GFAP in spinal cord tissues was up-regulated in group NP (P<0.05). Shh was mainly expressed in the cytoplasm of spinal dorsal horn neurons and in the gap around glial cells.
ConclusionShh signaling pathway in spinal cord is involved in the development and maintenance of NP in the rats.
Key words:
Hedgehog proteins; Signal transduction; Neuralgia; Spinal cord
Contributor Information
Xiaoxue Feng
Department of Anesthesiology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Danyan Liu
Department of Anesthesiology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Xiaoqiu Yang
Department of Anesthesiology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China