Effect of intrathecal morphine preconditioning on expression of nerve growth factor in dorsal root ganglia in a rat model of myocardial ischemia-reperfusion

Xu Shijin; He Shufang; Hu Jun; Huang Cheng; Zhang Ye

doi:10.3760/cma.j.issn.0254-1416.2016.06.005

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• 麻醉并发症 •

鞘内吗啡预处理对心肌缺血再灌注大鼠背根神经节神经生长因子表达的影响

中华麻醉学杂志, 2016,36(6) : 666-669. DOI: 10.3760/cma.j.issn.0254-1416.2016.06.005

摘要

目的

探讨鞘内吗啡预处理对心肌缺血再灌注大鼠背根神经节神经生长因子(NGF)表达的影响。

方法

鞘内置管成功的健康成年雄性SD大鼠30只，体重250～350 g，采用随机数字表法分为5组(n＝6)：假手术组(S组)、心肌缺血再灌注组(I/R组)、鞘内吗啡预处理组(ITMP组)、μ受体阻断剂CTOP＋鞘内吗啡预处理组(CTOP＋ITMP组)和CTOP对照组(CTOP组)。ITMP组缺血再灌注前30 min，经鞘内导管微量泵恒速输注吗啡3 μg/kg (10 μl) 5 min，间断5 min，重复3次；CTOP＋ITMP组和CTOP组分别于吗啡预处理前10 min和缺血前40 min鞘内注射1 μg/μl CTOP 10 μl。于再灌注120 min时处死大鼠，取心肌组织，测定心肌梗死体积；取背根神经节，分别采用免疫组化法和Western blot法检测NGF的表达。

结果

与S组比较，I/R组心肌梗死体积增大，背根神经节NGF表达上调(P<0.05)；与I/R组比较，ITMP组心肌梗死体积减小，背根神经节NGF表达下调(P<0.05)，CTOP组上述指标差异无统计学意义(P>0.05)；与ITMP组比较，CTOP＋ITMP组和CTOP组心肌梗死体积增大，背根神经节NGF表达上调(P<0.05)。

结论

鞘内吗啡预处理减轻大鼠心肌缺血再灌注损伤的机制与激活脊髓μ受体，抑制背根神经节NGF的表达，减轻伤害性刺激反应有关。

引用本文: 许士进, 何淑芳, 胡军, 等. 鞘内吗啡预处理对心肌缺血再灌注大鼠背根神经节神经生长因子表达的影响 [J] . 中华麻醉学杂志,2016,36 (6): 666-669. DOI: 10.3760/cma.j.issn.0254-1416.2016.06.005

参考文献导出: Endnote NoteExpress RefWorks NoteFirst 医学文献王

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鞘内吗啡预处理可减轻大鼠心肌缺血再灌注损伤^[1]，明确其机制具有一定意义。神经生长因子(NGF)是神经元存活和功能维持所必需的营养因子，研究表明，NGF除具有神经营养作用外，还可增强初级传入神经元对伤害性刺激的敏感性，在感觉神经元感受伤害性刺激过程中发挥重要作用^[2]。心肌缺血再灌注时NGF参与了心肌缺血后神经应激反应^[3]。背根神经节NGF表达增加，可增强伤害性刺激诱发的交感神经兴奋，导致心肌内儿茶酚胺类水平升高，这在短时期内对心脏有益，但交感神经过度兴奋会增加心肌氧耗，降低心肌对缺血再灌注伤害性刺激的耐受性，从而加重心肌细胞损伤^[4]。本研究拟探讨鞘内吗啡预处理对心肌缺血再灌注大鼠背根神经节NGF表达的影响。

贡献者信息

许士进

230601　合肥市，安徽医科大学第二附属医院麻醉科

何淑芳

230601　合肥市，安徽医科大学第二附属医院麻醉科

胡军

230601　合肥市，安徽医科大学第二附属医院麻醉科

黄成

230601　合肥市，安徽医科大学第二附属医院麻醉科

张野

230601　合肥市，安徽医科大学第二附属医院麻醉科

通信作者

张野

230601　合肥市，安徽医科大学第二附属医院麻醉科

Email：zhangye_hassan@sina.com

关键词

吗啡; 注射，脊髓; 缺血预处理; 神经生长因子; 心肌再灌注损伤; 神经节，脊;

基金项目

国家自然科学基金面上项目（81471145）

历史

出版日期：2016-06-20

收稿日期：2015-08-05

本文编辑

周晓云

Complication of Anesthesia

Effect of intrathecal morphine preconditioning on expression of nerve growth factor in dorsal root ganglia in a rat model of myocardial ischemia-reperfusion

Xu Shijin, He Shufang, Hu Jun, Huang Cheng, Zhang Ye

Published 2016-06-20

Cite as Chin J Anesthesiol, 2016,36(6): 666-669. DOI: 10.3760/cma.j.issn.0254-1416.2016.06.005

Abstract

Objective

To investigate the effect of intrathecal morphine preconditioning on the expression of nerve growth factor (NGF) in the dorsal root ganglia (DRG) in a rat model of myocardial ischemia-reperfusion (I/R).

Methods

Thirty healthy adult male Sprague-Dawley rats in which intrathecal catheters were successfully placed without complications, weighing 250-350 g, were randomly divided into 5 groups (n=6 each) using a random number table: sham operation group (S group), I/R group, intrathecal morphine preconditioning group (ITMP group), μ receptor antagonist CTOP + intrathecal morphine preconditioning group (CTOP + ITMP group), and CTOP control group (CTOP group). Myocardial ischemia was induced by 30 min of occlusion of the anterior descending branch of the left coronary artery followed by 120 min of reperfusion in all the groups except S group.Intrathecal morphine preconditioning was produced by 3 cycles of 5 min intrathecal injection of morphine 3 μg/kg (10 μl) at 5 min intervals within 30 min before ischemia in ITMP group.In CTOP+ ITMP and CTOP groups, 1 μg/μl CTOP 10 μl was injected intrathecally at 10 min before morphine preconditioning and 40 min before ischemia, respectively.At 120 min of reperfusion, the rats were sacrificed, and myocardial specimens were obtained for determination of myocardial infarct size, and DRGs were removed for determination of the expression of NGF by using immunohistochemistry and Western blot.

Results

Compared with S group, the myocardial infarct size was significantly increased, and the expression of NGF in DRGs was significantly up-regulated in I/R group (P<0.05). Compared with I/R group, the myocardial infarct size was significantly decreased, and the expression of NGF in DRGs was significantly down-regulated in ITMP group (P<0.05), and no significant change was found in the parameters mentioned above in CTOP group (P>0.05). Compared with ITMP group, the myocardial infarct size was significantly increased, and the expression of NGF in DRGs was significantly up-regulated in CTOP+ ITMP and CTOP groups (P<0.05).

Conclusion

The mechanism by which intrathecal morphine preconditioning reduces myocardial I/R injury is related to activation of spinal μ receptors, inhibition of NGF expression in DRGs, and reduction of responses to noxious stimulation in the rats.

Key words:

Morphine; Injection, spinal; Ischemic preconditioning; Nerve growth factor; Myocardial reperfusion injury; Ganglia, spinal

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Xu Shijin