Pain Management and Research
Changes in expression of spinal aquaporin-4 during remifentanil-induced hyperalgesia in a mouse model of incisional pain
Wu Biling, Lai Zhongmeng, Chen Wenhua, Zhang Liangcheng, Lin Pengtao
Published 2016-12-20
Cite as Chin J Anesthesiol, 2016,36(12): 1462-1464. DOI: 10.3760/cma.j.issn.0254-1416.2016.12.013
Abstract
ObjectiveTo evaluate the changes in the expression of spinal aquaporin-4 (AQP4) during remifentanil-induced hyperalgesia in a mouse model of incisional pain.
MethodsSeventy-two pathogen-free healthy adult male CD1 mice, weighing 25-30 g, were divided into 4 groups (n=18 each) using a random number table: control group (group C), incisional pain (group I), remifentanil group (group R) and remifentanil plus incisional pain group (group R+ I). Normal saline was infused subcutaneously in group C. An incision was made in the left hind paw in group I. Remifentanil 80 μg/kg was subcutaneously infused for 30 min at a rate of 0.8 ml/h in group R. Remifentanil was infused subcutaneously before establishment of the model in group R+ I.The thermal paw withdrawal latency (TWL) and mechanical paw withdrawal threshold (MWT) were measured at 1 day before establishment of the model (T0) and 6 h and 1, 2 and 7 days after establishment of the model (T1-4). After measurement of the pain threshold at T3, 12 animals were sacrificed randomly, and the lumbar segment of the spinal cord was removed for determination of the distribution and expression of AQP4 by Western blot.
ResultsCompared with group C, the TWL was significantly shortened at T1-3, and the MWT was decreased at T2-4 in R and R + I groups, and the expression of AQP4 was significantly up-regulated at T3 in I, R and R+ I groups (P<0.05). Compared with group I, the TWL was significantly shortened at T2, 3, and the MWT was decreased at T2-4 in group R, and the TWL was significantly shortened at T1-3, the MWT was decreased at T2-4, and the expression of AQP4 was up-regulated at T3 in group R+ I (P<0.05).
ConclusionThe mechanism by which remifentanil induces hyperalgesia is related to up-regulation of AQP4 expression in the spinal cord in a mouse model of incisional pain.
Key words:
Piperidines; Pain, postoperative; Hyperalgesia; Spinal cord; Aquaporin 4
Contributor Information
Wu Biling
Department of Anesthesiology, Union Hospita1, Fujian Medical University, Fuzhou 350001, China
Lai Zhongmeng
Department of Anesthesiology, Union Hospita1, Fujian Medical University, Fuzhou 350001, China
Chen Wenhua
Department of Anesthesiology, Union Hospita1, Fujian Medical University, Fuzhou 350001, China
Zhang Liangcheng
Department of Anesthesiology, Union Hospita1, Fujian Medical University, Fuzhou 350001, China
Lin Pengtao
Department of Anesthesiology, Union Hospita1, Fujian Medical University, Fuzhou 350001, China
