Critical Care Medicine
Relationship between TLR4/NF-κB signaling pathway and propofol-induced inhibition of endotoxin-induced release of TNF-α from alveolar macrophages of rats
Yang Xue, Sun Jiu, Zeng Si, Lan Zhixun
Published 2017-06-20
Cite as Chin J Anesthesiol, 2017,37(06): 761-764. DOI: 10.3760/cma.j.issn.0254-1416.2017.06.032
Abstract
ObjectiveTo evaluate the relationship between Toll-like receptor 4(TLR4)/nuclear factor kappa B(NF-κB)signaling pathway and propofol-induced inhibition of endotoxin-induced release of tumor necrosis factor-alpha(TNF-α)from alveolar macrophages(AMs)of rats.
MethodsAMs extracted from adult male Sprague-Dawley rats were cultured and inoculated in 6-well plates(1×106 cells/well)and in 96-well plates(1×104 cells/well). The cells were divided into 5 groups(n=18 each)using a random number table: control group(group C), dimethyl sulfoxide group(group D), lipopolysaccharide(LPS)group(group L), propofol group(group P)and LPS plus propofol group(group L+ P). The cells were continuously cultured with phosphate buffer solution in group C. Dimethyl sulfoxide was added at the final concentration of 5 mg/ml in group D. LPS was added at the final concentration of 1 μg/ml in group L. Propofol was added at the final concentration of 25 μmol/L(4.46 μg/ml)in group P. LPS and propofol were added at the final concentration of 1 μg/ml and 25 μmol/L(4.46 μg/ml), respectively, in group L+ P.At 24 h of culture or incubation, the cell viability was detected by CCK-8 assay, the morphological changes of cells were observed using Wright′s staining, the concentration of TNF-α in the supernatant was determined by enzyme-linked immunosorbent assay, and TLR4 expression and NF-κB activities were measured by Western blot.
ResultsCompared with group C, the cell viability and concentration of TNF-α in the supernatant were significantly increased, the expression of TLR4 was up-regulated, and the activity of NF-κB was enhanced in L and L+ P groups(P<0.05), and no significant change was found in the parameters mentioned above in D and P groups(P>0.05). Compared with group L, the cell viability and concentration of TNF-α in the supernatant were significantly decreased, the expression of TLR4 was down-regulated, and the activity of NF-κB was weakened(P<0.05), the morphological changes of cells were significantly attenuated, and the number of pseudopodia was reduced in group L+ P.
ConclusionThe mechanism by which propofol inhibits endotoxin-induced release of TNF-α from AMs is related to inhibited activation of TLR4/NF-κB signaling pathway in rats.
Key words:
Toll-like receptor 4; NF-kappa B; Propofol; Endotoxins; Macrophages, alveolar; Tumor necrosis factor-alpha
Contributor Information
Yang Xue
Department of Anesthesiology, First People′s Hospital of Yibin, Yibin 644000, Sichuan Province, China
Sun Jiu
Department of Anesthesiology, First People′s Hospital of Yibin, Yibin 644000, Sichuan Province, China
Zeng Si
Department of Anesthesiology, Sichuan Provincal People′s Hospital, Chengdu 610000, China
Lan Zhixun
Department of Anesthesiology, Sichuan Provincal People′s Hospital, Chengdu 610000, China
