Critical Care Medicine
Effect of Shenfu injection preconditioning on ventilator-induced brain injury in rats
Ke Luo, Luojing Zhou, Cheng Chen, Weizhen Guo, Yujuan Wu, Yali Ge, Ju Gao
Published 2018-04-20
Cite as Chin J Anesthesiol, 2018, 38(4): 481-483. DOI: 10.3760/cma.j.issn.0254-1416.2018.04.026
Abstract
ObjectiveTo investigate the effect of Shenfu injection preconditioning on ventilator-induced brain injury in rats.
MethodsSixty healthy adult male Sprague-Dawley rats, weighing 300-320 g, were divided into 3 groups (n=20 each) using a random number table: control group (group C), ventilator-induced brain injury group (group VIBI) and Shenfu injection group (group SF). Rats were mechanically ventilated for 6 h with tidal volume of 40 ml/kg in VIBI and SF groups.Rats were mechanically ventilated for 6 h with tidal volume of 10 ml/kg in group C. Shenfu injection 20 ml/kg was injected intraperitoneally at 30 min before ventilation in group SF.Blood samples were collected at 2 and 6 h of ventilation for measurement of the concentrations of serum S100β protein (by enzyme-linked immunosorbent assay). Then the rats were sacrificed at the end of ventilation, and brains were removed for determination of the contents of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) (by enzyme-linked immunosorbent assay) and expression of glutamate decarboxylase (GAD) and γ-aminobutyric acid type A (GABAA) receptor in brain tissues (by Western blot).
ResultsCompared with group C, the serum concentrations of S100β protein at each time point of ventilation and contents of IL-1β and TNF-α in brain tissues were significantly increased, and the expression of GAD and GABAA receptor was down-regulated in VIBI and SF groups (P<0.05). Compared with group VIBI, the serum concentrations of S100β protein at each time point of ventilation and contents of IL-1β and TNF-α in brain tissues were significantly decreased, and the expression of GAD and GABAA receptor was up-regulated in group SF (P<0.05).
ConclusionShenfu injection preconditioning can relieve ventilator-induced brain injury in rats, and the mechanism may be related to inhibiting inflammatory responses and activating GABA signaling pathway.
Key words:
SHENFU DECOCTION; Respiration, artificial; Brain injuries
Contributor Information
Ke Luo
Department of Anesthesiology, Clinical Medical School of Yangzhou University (Subei People′s Hospital of Jiangsu Province), Yangzhou 225001, China
Luojing Zhou
Department of Science and Education, Clinical Medical School of Yangzhou University (Subei People′s Hospital of Jiangsu Province), Yangzhou 225001, China
Cheng Chen
Department of Anesthesiology, Clinical Medical School of Yangzhou University (Subei People′s Hospital of Jiangsu Province), Yangzhou 225001, China
Weizhen Guo
Department of Anesthesiology, Clinical Medical School of Yangzhou University (Subei People′s Hospital of Jiangsu Province), Yangzhou 225001, China
Yujuan Wu
Department of Anesthesiology, Clinical Medical School of Yangzhou University (Subei People′s Hospital of Jiangsu Province), Yangzhou 225001, China
Yali Ge
Department of Anesthesiology, Clinical Medical School of Yangzhou University (Subei People′s Hospital of Jiangsu Province), Yangzhou 225001, China
Ju Gao
Department of Anesthesiology, Clinical Medical School of Yangzhou University (Subei People′s Hospital of Jiangsu Province), Yangzhou 225001, China