Critical Care Medicine
Role of HSP70 in ozone preconditioning-induced reduction of hepatic ischemia-reperfusion injury in rats
Weixin Zhong, Lianghui Li, Lanlan Wang, Wenhua Chen
Published 2018-04-20
Cite as Chin J Anesthesiol, 2018, 38(4): 493-496. DOI: 10.3760/cma.j.issn.0254-1416.2018.04.029
Abstract
ObjectiveTo evaluate the role of heat shock protein 70 (HSP70) in ozone preconditioning-induced reduction of hepatic ischemia-reperfusion (I/R) injury in rats.
MethodsTwenty-four pathogen-free healthy male Sprague-Dawley rats, aged 8 weeks, weighing 250-300 g, were divided into 4 groups (n=6 each) using a random number table: sham operation group (group S), group I/R, ozone preconditioning group (group OP) and HSP70 inhibitor quercetin group (group Q). Hepatic I/R injury was induced by clamping the branches of hepatic artery supplying left and middle lobes of the liver, portal vein and bile vessel for 45 min, followed by 2 h of reperfusion.In group OP, the mixture of ozone and oxygen was intraperitoneally injected at the concentration of ozone 50 μg/ml and bolus dose 1 mg/kg once a day for 5 consecutive days before operation.In group Q, quercetin 7 mg/kg was intraperitoneally injected at 1 h before injecting the mixture of ozone and oxygen.Blood samples from the abdominal aorta were collected at 2 h of reperfusion for determination of serum alanine transaminase (ALT) and aspartate transaminase (AST) activities and concentrations of interleukin-1beta (IL-1β), IL-6 and tumor necrosis factor-alpha (TNF-α) in serum (by enzyme-linked immunosorbent assay). The animals were sacrificed after blood sampling, and hepatic tissue specimens were collected for detection of activated caspase-3 expression (by Western blot) and cell apoptosis (by TUNEL). Apoptotic index (AI) was calculated.
ResultsCompared with group S, the serum ALT and AST activities, concentrations of IL-1β, IL-6 and TNF-α in serum and AI were significantly increased, and the expression of activated caspase-3 was up-regulated in group I/R (P<0.05 or 0.01). Compared with group I/R, the serum ALT and AST activities, concentrations of IL-1β, IL-6 and TNF-α in serum and AI were significantly decreased, and the expression of activated caspase-3 was down-regulated in group OP (P<0.05 or 0.01). Compared with group OP, the serum ALT and AST activities, concentrations of IL-1β, IL-6 and TNF-α in serum and AI were significantly increased, and the expression of activated caspase-3 was up-regulated in group Q (P<0.05 or 0.01).
ConclusionThe mechanism by which ozone preconditioning reduces hepatic I/R injury is related to activating HSP70 and inhibiting inflammatory responses and cell apoptosis in rats.
Key words:
Ozone; Liver; Reperfusion injury; HSP70 heat-shock proteins
Contributor Information
Weixin Zhong
Department of Anesthesiology, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, China
Lianghui Li
Department of Anesthesiology, Southern Branch of Fujian Provincial Hospital, Fuzhou 350028, China
Lanlan Wang
Department of Anesthesiology, Fujian Provincial Cancer Hospital, Fuzhou 350001, China
Wenhua Chen
Department of Anesthesiology, Fujian Medical University Union Hospital, Fuzhou 350001, China