Pain Management and Research
Role of spinal CCR5 in remifentanil-induced hyperalgesia in rats with incisional pain
Nan Li, Xin Wang, Linlin Zhang, Ruichen Shu, Suqian Guo, Qi Zhao, Guolin Wang
Published 2018-05-20
Cite as Chin J Anesthesiol, 2018, 38(5): 575-578. DOI: 10.3760/cma.j.issn.0254-1416.2018.05.018
Abstract
ObjectiveTo evaluate the role of spinal C-C motif chemokine receptor 5 (CCR5) in remifentanil-induced hyperalgesia in rats with incisional pain.
MethodsThirty-two male Sprague-Dawley rats, weighing 240-260 g, aged 2-3 months, in which intrathecal and caudal catheters were successfully implanted, were divided into 4 groups (n=8 each) using a random number table: control group (group C), CCR5 antagonist maraviroc group (group M), remifentanil plus incisional pain group (group R+ I) and maraviroc plus remifentanil plus incisional pain group (group M+ R+ I). Phosphate buffer solution (PBS) 10 μl was intrathecally injected and normal saline was infused for 60 min at 1 μg·kg-1·min-1 via the caudal vein in group C. Maraviroc 100 pmol (in 10 μl of PBS) was intrathecally injected and normal saline was infused for 60 min at 1 μg·kg-1·min-1 via the caudal vein in group M. PBS 10 μl was intrathecally injected, then the model of incisional pain was established, and remifentanil 1 μg·kg-1·min-1 was infused for 60 min via the caudal vein in group R+ I.Maraviroc 100 pmol (in 10 μl of PBS) was intrathecally injected, then the model of incisional pain was established, and remifentanil 1 μg·kg-1·min-1 was infused for 60 min via the caudal vein in group M+ R+ I.The mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured at 24 h before infusion of remifentanil or normal saline (T0) and 2, 6, 24 and 48 h after stopping infusion (T1-4). The rats were sacrificed after the last measurement of pain threshold, and L4-6 segments of the spinal cord were removed for determination of the expression of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule-1 (Iba-1) by Western blot.
ResultsCompared with group C, the MWT was significantly decreased and TWL was shortened at T1-4, and the expression of GFAP and Iba-1 in the spinal cord was up-regulated in R+ I and M+ R+ I groups (P<0.05), and no significant change was found in the parameters mentioned above in group M (P>0.05). Compared with group R+ I, the MWT was significantly increased and TWL was prolonged at T1-4, and the expression of GFAP and Iba-1 in the spinal cord was down-regulated in group M+ R+ I (P<0.05).
ConclusionSpinal CCR5 is involved in remifentanil-induced hyperalgesia in the rats with incisional pain, and the mechanism may be related to activating astrocytes and microglias.
Key words:
Piperidines; Hyperalgesia; Pain, postoperative; Receptors, CCR5; Spinal cord
Contributor Information
Nan Li
Department of Anesthesiology, Tianjin Medical University General Hospital Tianjin Research Institute of Anesthesiology, Tianjin 300052, China
Xin Wang
Department of Anesthesiology, Tianjin Medical University General Hospital Tianjin Research Institute of Anesthesiology, Tianjin 300052, China
Linlin Zhang
Department of Anesthesiology, Tianjin Medical University General Hospital Tianjin Research Institute of Anesthesiology, Tianjin 300052, China
Ruichen Shu
Department of Anesthesiology, Tianjin Medical University General Hospital Tianjin Research Institute of Anesthesiology, Tianjin 300052, China
Suqian Guo
Department of Anesthesiology, Tianjin Medical University General Hospital Tianjin Research Institute of Anesthesiology, Tianjin 300052, China
Qi Zhao
Department of Anesthesiology, Tianjin Medical University General Hospital Tianjin Research Institute of Anesthesiology, Tianjin 300052, China
Guolin Wang
Department of Anesthesiology, Tianjin Medical University General Hospital Tianjin Research Institute of Anesthesiology, Tianjin 300052, China