Critical Care Medicine
Effect of resuscitation with Ringer′s malate solution on acute lung injury caused by hemorrhagic shock in rats
Xiaohong Chen, Yi Zhu, Wenli Gao, Qi Wang, Ping Wang, Shanglong Yao, Zhongliang Dai
Published 2018-07-20
Cite as Chin J Anesthesiol, 2018, 38(7): 874-877. DOI: 10.3760/cma.j.issn.0254-1416.2018.07.026
Abstract
ObjectiveTo evaluate the effect of resuscitation with Ringer′s malate solution on acute lung injury caused by hemorrhagic shock in rats.
MethodsForty-eight SPF healthy male Sprague-Dawley rats, aged 7-9 weeks, weighing 280-320 g, were assigned into 4 groups (n=12 each) using a random number table method: sham operation group (group S), normal saline group (group NS), Ringer′s lactate solution group (group RL) and Ringer′s malate solution group (group RM). In NS, RL and RM groups, the model of hemorrhagic shock was established, and rats were resuscitated after 60 min of hemorrhagic shock.Rats were sacrificed at 3 h after resuscitation, and bronchial alveolar lavage fluid (BALF) was collected to count neutrophils.Lung tissues were obtained for determination of the wet/dry weight ratio (W/D ratio), myeloperoxidase (MPO) activity, and contents of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) and IL-6.Lung tissues were obtained for examination of the pathological changes.
ResultsCompared with group S, the neutrophil count in BALF, W/D ratio, MPO activity and contents of TNF-α, IL-1β and IL-6 were significantly increased in NS, RL and RM groups (P<0.05). Compared with NS and RL groups, the neutrophil count in BALF, W/D ratio, MPO activity and contents of TNF-α, IL-1β and IL-6 were significantly decreased in NS and RL groups (P<0.05).
ConclusionThe severity of acute lung injury is reduced when Ringer′s malate solution is used for resuscitation as compared with that when normal saline and Ringer′s lactate solution are used in a rat model of hemorrhagic shock.
Key words:
Malates; Shock, hemorrhagic; Fluid therapy; Respiratory distress syndrome, adult
Contributor Information
Xiaohong Chen
Department of Anesthesiology, Maternal and Child Health Hospital of Hainan Province, Haikou 570206, China
Yi Zhu
Department of Anesthesiology, Shenzhen Anesthesiology Engineering Center, Shenzhen People′s Hospital, The Second Medical College of Jinan University, Shenzhen 518020, China
Wenli Gao
Department of Anesthesiology, Shenzhen Anesthesiology Engineering Center, Shenzhen People′s Hospital, The Second Medical College of Jinan University, Shenzhen 518020, China
Qi Wang
Department of Anesthesiology, Shenzhen Anesthesiology Engineering Center, Shenzhen People′s Hospital, The Second Medical College of Jinan University, Shenzhen 518020, China
Ping Wang
Department of Anesthesiology, Shenzhen Anesthesiology Engineering Center, Shenzhen People′s Hospital, The Second Medical College of Jinan University, Shenzhen 518020, China
Shanglong Yao
Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhoung University of Science and Technology, Wuhan 430022, China
Zhongliang Dai
Department of Anesthesiology, Shenzhen Anesthesiology Engineering Center, Shenzhen People′s Hospital, The Second Medical College of Jinan University, Shenzhen 518020, China
